Wettability gradient on the elytra in the aquatic beetle Cybister chinensis and its role in angular position of the beetle at water-air interface

The surface of the elytra in some species of aquatic beetles displays relatively low contact angles (CAs), even showing hydrophilic properties. In this study, we report on an observation that both sexes of Cybister chinensis beetle fresh elytral surface do not exhibit uniform CA, but rather a wettability gradient along the longitudinal axis in posterior direction. The wettability is very different between females and males due to the presence (female) or absence (male) of channels on the elytral surface. When a small drop of water touches the elytra surface, it tends to slide towards the anterior having a lower CA on the elytra. This gradient presumably supports a breathing-associated behavior of beetles in which they cause the tip of their abdomen to protrude into the surface of the water in order to collect an air bubble for oxygen uptake and, when floating on the surface, to keep the body inclined at a small angle to the water's surface with their heads immersed. Statement of Significance Hydrophobicity on surfaces is a fundamental property which has attracted great interest across all scientific disciplines, here we have demonstrated that the gradually changing chemistry of the elytral surface facilitates the tilted beetle posture on the water's surface. The mechanism of water interacting with the elytra demonstrated the most energetically favorable posture in the diving beetles. Surfaces with directional wetting properties that promote droplet drainage are of significant practical importance in many fields. The anisotropic topography and wetting properties of the elytra may inspire microfluidic devices for medical and robotic applications.