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Unlabeled flow cellular deformation measurement based on digital holographic microscopy

  • Wen Xiao
  • , Qixiang Wang*
  • , Feng Pan
  • , Runyu Cao
  • , Xiaosu Yi
  • *此作品的通讯作者
  • Beihang University

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

Living cells as phase objects require not only non-invasive measurement but also quantitative phase information during dynamic biopsy. Digital Holographic Microscopy (DHM), measuring three-dimensional morphology without changing the active condition of cells and in situ inspection, is becoming excellent tools for biology research. We have described a DHM method for quantitative, unlabeled observation of living cell subjected to fluid shear stress (FSS) in flowing fluid. The holographic recording system combined with the fluid shear system is improved. The numerical reconstruction technique firstly employed deep learning Convolutional Neural Network model filter, which achieved automatically processing large scale the spectrum of holograms immediately. Osteocytes as the experimental samples were observed and their morphological changes under the stimulation of FSS was successfully measured.

源语言英语
主期刊名Interferometry XIX
编辑Katherine Creath, Jan Burke, Angela D. Davies, Michael B. North Morris, Katherine Creath
出版商SPIE
ISBN(电子版)9781510620698
DOI
出版状态已出版 - 2018
活动Interferometry XIX 2018 - San Diego, 美国
期限: 21 8月 201823 8月 2018

出版系列

姓名Proceedings of SPIE - The International Society for Optical Engineering
10749
ISSN(印刷版)0277-786X
ISSN(电子版)1996-756X

会议

会议Interferometry XIX 2018
国家/地区美国
San Diego
时期21/08/1823/08/18

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