Two-photon laser scanning stereomicroscopy for fast volumetric imaging

Yanlong Yang; Baoli Yao; Ming Lei; Dan Dan; Runze Li; Mark Van Horn; Xun Chen; Yang Li; Tong Ye

doi:10.1371/journal.pone.0168885

Two-photon laser scanning stereomicroscopy for fast volumetric imaging

Yanlong Yang
, Baoli Yao
, Ming Lei
, Dan Dan
, Runze Li
, Mark Van Horn
, Xun Chen
, Yang Li
, Tong Ye

CAS - Xi'an Institute of Optics and Precision Mechanics
Medical University of South Carolina
Clemson University

科研成果: 期刊稿件 › 文章 › 同行评审

17 引用（Scopus）

摘要

Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

源语言	英语
文章编号	e0168885
期刊	PLOS ONE
卷	11
期	12
DOI	https://doi.org/10.1371/journal.pone.0168885
出版状态	已出版 - 12月 2016
已对外发布	是

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abstract = "Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.",

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AU - Yang, Yanlong

AU - Yao, Baoli

AU - Lei, Ming

AU - Dan, Dan

AU - Li, Runze

AU - Van Horn, Mark

AU - Chen, Xun

AU - Li, Yang

AU - Ye, Tong

N1 - Publisher Copyright: © This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

PY - 2016/12

Y1 - 2016/12

N2 - Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

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Two-photon laser scanning stereomicroscopy for fast volumetric imaging

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