Stimulated Raman spectroscopic imaging by microsecond delay-line tuning

Chien Sheng Liao; Kai Chih Huang; Weili Hong; Andy J. Chen; Caroline Karanja; Pu Wang; Gregory Eakins; Ji Xin Cheng

doi:10.1364/OPTICA.3.001377

Stimulated Raman spectroscopic imaging by microsecond delay-line tuning

Chien Sheng Liao
, Kai Chih Huang
, Weili Hong
, Andy J. Chen
, Caroline Karanja
, Pu Wang
, Gregory Eakins
, Ji Xin Cheng^*

^*此作品的通讯作者

Purdue University

科研成果: 期刊稿件 › 快报 › 同行评审

90 引用（Scopus）

摘要

Stimulated Raman scattering (SRS) microscopy is an emerging platform for vibrational imaging of living systems. We present microsecond-scale SRS spectroscopic imaging by temporally tuning two spectrally focused pulses through a resonant delay line. Our platform is able to acquire an SRS spectrum in 42 μs and form a spectral image of 40,000 pixels within 3.3 s. Spectroscopic identification of single bacteria and fungi in blood and 4-D imaging (x-y-z-λ) of intracellular organelles in live C. elegans are demonstrated.

源语言	英语
页（从-至）	1377-1380
页数	4
期刊	Optica
卷	3
期	12
DOI	https://doi.org/10.1364/OPTICA.3.001377
出版状态	已出版 - 20 12月 2016
已对外发布	是

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title = "Stimulated Raman spectroscopic imaging by microsecond delay-line tuning",

abstract = "Stimulated Raman scattering (SRS) microscopy is an emerging platform for vibrational imaging of living systems. We present microsecond-scale SRS spectroscopic imaging by temporally tuning two spectrally focused pulses through a resonant delay line. Our platform is able to acquire an SRS spectrum in 42 μs and form a spectral image of 40,000 pixels within 3.3 s. Spectroscopic identification of single bacteria and fungi in blood and 4-D imaging (x-y-z-λ) of intracellular organelles in live C. elegans are demonstrated.",

keywords = "Nonlinear microscopy, Raman spectroscopy",

author = "Liao, \{Chien Sheng\} and Huang, \{Kai Chih\} and Weili Hong and Chen, \{Andy J.\} and Caroline Karanja and Pu Wang and Gregory Eakins and Cheng, \{Ji Xin\}",

note = "Publisher Copyright: {\textcopyright} 2016 Optical Society of America.",

year = "2016",

month = dec,

day = "20",

doi = "10.1364/OPTICA.3.001377",

language = "英语",

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TY - JOUR

T1 - Stimulated Raman spectroscopic imaging by microsecond delay-line tuning

AU - Liao, Chien Sheng

AU - Huang, Kai Chih

AU - Hong, Weili

AU - Chen, Andy J.

AU - Karanja, Caroline

AU - Wang, Pu

AU - Eakins, Gregory

AU - Cheng, Ji Xin

PY - 2016/12/20

Y1 - 2016/12/20

N2 - Stimulated Raman scattering (SRS) microscopy is an emerging platform for vibrational imaging of living systems. We present microsecond-scale SRS spectroscopic imaging by temporally tuning two spectrally focused pulses through a resonant delay line. Our platform is able to acquire an SRS spectrum in 42 μs and form a spectral image of 40,000 pixels within 3.3 s. Spectroscopic identification of single bacteria and fungi in blood and 4-D imaging (x-y-z-λ) of intracellular organelles in live C. elegans are demonstrated.

AB - Stimulated Raman scattering (SRS) microscopy is an emerging platform for vibrational imaging of living systems. We present microsecond-scale SRS spectroscopic imaging by temporally tuning two spectrally focused pulses through a resonant delay line. Our platform is able to acquire an SRS spectrum in 42 μs and form a spectral image of 40,000 pixels within 3.3 s. Spectroscopic identification of single bacteria and fungi in blood and 4-D imaging (x-y-z-λ) of intracellular organelles in live C. elegans are demonstrated.

KW - Nonlinear microscopy

KW - Raman spectroscopy

UR - https://www.scopus.com/pages/publications/85007071362

U2 - 10.1364/OPTICA.3.001377

DO - 10.1364/OPTICA.3.001377

M3 - 快报

AN - SCOPUS:85007071362

SN - 2334-2536

VL - 3

SP - 1377

EP - 1380

JO - Optica

JF - Optica

IS - 12

ER -

Stimulated Raman spectroscopic imaging by microsecond delay-line tuning

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