TY - JOUR
T1 - Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos
AU - Jiang, Lan
AU - Zhang, Jing
AU - Wang, Jing Jing
AU - Wang, Lu
AU - Zhang, Li
AU - Li, Guoqiang
AU - Yang, Xiaodan
AU - Ma, Xin
AU - Sun, Xin
AU - Cai, Jun
AU - Zhang, Jun
AU - Huang, Xingxu
AU - Yu, Miao
AU - Wang, Xuegeng
AU - Liu, Feng
AU - Wu, Chung I.
AU - He, Chuan
AU - Zhang, Bo
AU - Ci, Weimin
AU - Liu, Jiang
PY - 2013/5/9
Y1 - 2013/5/9
N2 - 5-methylcytosine is a major epigenetic modification that is sometimes called "the fifth nucleotide." However, our knowledge of how offspring inherit the DNA methylome from parents is limited. We generated nine single-base resolution DNA methylomes, including zebrafish gametes and early embryos. The oocyte methylome is significantly hypomethylated compared to sperm. Strikingly, the paternal DNA methylation pattern is maintained throughout early embryogenesis. The maternal DNA methylation pattern is maintained until the 16-cell stage. Then, the oocyte methylome is gradually discarded through cell division and is progressively reprogrammed to a pattern similar to that of the sperm methylome. The passive demethylation rate and the de novo methylation rate are similar in the maternal DNA. By the midblastula stage, the embryo's methylome is virtually identical to the sperm methylome. Moreover, inheritance of the sperm methylome facilitates the epigenetic regulation of embryogenesis. Therefore, besides DNA sequences, sperm DNA methylome is also inherited in zebrafish early embryos.
AB - 5-methylcytosine is a major epigenetic modification that is sometimes called "the fifth nucleotide." However, our knowledge of how offspring inherit the DNA methylome from parents is limited. We generated nine single-base resolution DNA methylomes, including zebrafish gametes and early embryos. The oocyte methylome is significantly hypomethylated compared to sperm. Strikingly, the paternal DNA methylation pattern is maintained throughout early embryogenesis. The maternal DNA methylation pattern is maintained until the 16-cell stage. Then, the oocyte methylome is gradually discarded through cell division and is progressively reprogrammed to a pattern similar to that of the sperm methylome. The passive demethylation rate and the de novo methylation rate are similar in the maternal DNA. By the midblastula stage, the embryo's methylome is virtually identical to the sperm methylome. Moreover, inheritance of the sperm methylome facilitates the epigenetic regulation of embryogenesis. Therefore, besides DNA sequences, sperm DNA methylome is also inherited in zebrafish early embryos.
UR - https://www.scopus.com/pages/publications/84877696605
U2 - 10.1016/j.cell.2013.04.041
DO - 10.1016/j.cell.2013.04.041
M3 - 文章
C2 - 23663777
AN - SCOPUS:84877696605
SN - 0092-8674
VL - 153
SP - 773
EP - 784
JO - Cell
JF - Cell
IS - 4
ER -