Relationship between serum miR-27 and lower extremity vascular disease and 25 (OH) D3 level in elderly patients with type 2 diabetes mellitus

Objective To analyze the relationship between serum miR 27 and lower extremity vascular disease and 25 (OH) D3 level in elderly patients with type 2 diabetes mellitus(T2DM). Methods From October 2017 to May 2019, 86 elderly patients with type 2 diabetes diagnosed by the Department of endocrinology7 of Cangzhou people xs Hospital of Hebei Province were selected as T2DM group. The patients with or without lower extremity vascular disease were divided into lower extremity vascular disease (LLPAD) sub group (44 cases) and non-lower extremity vascular disease (Non-LLPAD) sub group (42 cases). 76 cases of healthy elderly physical examination were selected as the control group. The expression of miR-27 mRNA and the levels of TC, TG, HDL-C, LDL-C, HbAlc, FPG, 25 (OH) D3, hs-CRP and Fib were measured and compared. Results The levels of miR-27 mRNA, TC, TG, LDL-C, Fib, HbAlc, hs-CRP, and FPG were higher in the T2DM group than in the control group, and 25 (OH) D3 and HDL-C were lower than those in the control group (t = 13. 371, 13.036,25.364,19.698,29.326,38.489,95.524,66.547,66.241,8.698,P=0.000). The levels of miR-27 mRNA, TC, TG, LDL-C, Fib, HbAlc, hs-CRP, and FPG in the LLPAD subgroup were higher than those in the Non-LLPAD subgroup, and the levels of 25 (OH) D₃and HDL-C were lower than those in the Non-LLPAD subgroup (i =5. 275, 13. 541, 26. 541, 8.965, 20.146, 14.548, 14.369, 23.654, 7. 711, 9.491, allP =0. 000). miR-27 mRNA was negatively correlated with 25 (OH) D3 and HDL-C (r =-0. 486,-0. 483, P = 0. 039, 0. 039), and positively correlated with TC, TG, LDL-C, Fib, HbA_lchs-CRP, FPG (r=0.651, 0.572, 0.494, 0.747, 0.438, 0.523, 0.591, P<0.05). High levels of miR-27 mRNA,TC and low levels of 25 (OH) D₃were all risk factors for senile type 2 diabetes with lower extremity vascular disease (OR = 2.437, 4. 268, 13. 625, P < 0. 05). The AUCs of miR-27 mRNA and 25 (OH) D₃were similar, and were smaller than TC. The sensitivity and specificity of miR-27 mRNA and 25 (OH) D3 in the diagnosis of lower extremity vascular disease in elderly patients with type 2 diabetes mellitus were similar (P > 0. 05). Conclusion The level of serum miR-27 mRNA in elderly patients with type 2 diabetic lower extremity angiopathy was increased, which was negatively correlated with 25 (OH) D3. miR-27 and 25 (OH) D3 had high sensitivity and specificity in the diagnosis of type 2 diabetic lower extremity angiopathy, which could be used as biomarkers for the determination of type 2 diabetic lower extremity angiopathy.