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Rapid autofocusing for dynamic observation exploiting holographic polarization microscope

  • Beihang University
  • National Research Council of Italy

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

We proposed a rapid autofocusing method exploiting holographic polarization microscope, which could determine the refocusing distance without multiple reconstructions and complex network training. The defocus distance of the object is calculated rapidly by identifying the separation distance of the two defocus images of the object and using the linear relation between the defocus distance of the object and the separation distance of the two defocus images of the object. The linear relationship is obtained by numerical propagation and linear fitting. Last, we have demonstrated the effectiveness of the proposed method by reconstructing the in-focus images of flowing cells. We believe that our proposed method could be a powerful tool for dynamic observation and extend the application of holographic polarization microscope.

源语言英语
主期刊名Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V
编辑Pietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Christoph K. Hitzenberger
出版商SPIE
ISBN(电子版)9781510644069
DOI
出版状态已出版 - 2021
活动Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 - Virtual, Online, 德国
期限: 21 6月 202125 6月 2021

出版系列

姓名Proceedings of SPIE - The International Society for Optical Engineering
11786
ISSN(印刷版)0277-786X
ISSN(电子版)1996-756X

会议

会议Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021
国家/地区德国
Virtual, Online
时期21/06/2125/06/21

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