Optoelectronic Navigation-Based Microtruck: For Efficient Cargo Loading, Transport, and Unloading

This study proposes an optoelectronic navigation strategy leveraging Ag-SiO₂ microspheres as 'microtruck' to overcome the limitations of traditional optoelectronic tweezers (OET) in manipulating negative dielectrophoresis (nDEP) particles. By dynamically adjusting electric field frequency and optical parameters, we regulate particle-induced dielectrophoretic forces (PiDEP) to achieve efficient adsorption, high-speed transport, and site-specific unloading of nDEP-responsive cargo. Experimental results demonstrate a seven times enhancement in manipulation velocity compared to conventional direct optical methods, along with the capability for simultaneous multi-particle transport. In addition, we utilized finite element simulations to analyze the optimal electric field frequency and optical parameters for the microtruck's loading and unloading processes. Furthermore, a systematic analysis of critical velocities and failure modes under varying cargo loads further validates the robustness of this approach. Demonstrated within a labyrinthine microenvironment, this strategy enables programmable navigation, sequential cargo handling, and micrometer positional accuracy. This study provides an efficient solution for biomedical applications, including precise single-cell manipulation and targeted drug delivery.