TY - JOUR
T1 - Mechano growth factor pretreatment yield mechanical stimuli induced cell stress responses in ligament fibroblasts of osteoarthritis via activating ATF-2
AU - Ma, Yu
AU - Song, Yang
AU - Li, Linhao
AU - Dong, Lili
AU - Wang, Chunli
AU - Wang, Pingping
AU - Yang, Li
N1 - Publisher Copyright:
© 2020, Springer Nature B.V.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Objective: The purpose of this study is to investigate whether mechanical growth factor (MGF) promotes mechanical response to ligament fibroblasts in osteoarthritis knee cavity via activating transcription factor 2 (ATF-2). Results: Osteoarthritis ligament fibroblasts (OA-LFs) were suffered from 12% static mechanical stretch to mimic mechanical force mediated ligament injury. Meanwhile, OA-LFs were treated with MGF before and during mechanical stretch. We observed that OA delayed LFs response to mechanical injury, while MGF pretreatment promoted cells timely feedback the mechanically stimuli by inducing cellular stress. Additionally, MGF accelerated the ligament injury repair by promoting cell migration, decreasing the MMP-2 activity, and remitting the cell deformation via ATF-2 activating in cells. Conclusions: Our study shows that MGF pretreatment of OA-LFs can respond quickly to mechanical damage and repair ligament tissue by activating ATF-2. Therefore, MGF has potential as a therapeutic for OA patients.
AB - Objective: The purpose of this study is to investigate whether mechanical growth factor (MGF) promotes mechanical response to ligament fibroblasts in osteoarthritis knee cavity via activating transcription factor 2 (ATF-2). Results: Osteoarthritis ligament fibroblasts (OA-LFs) were suffered from 12% static mechanical stretch to mimic mechanical force mediated ligament injury. Meanwhile, OA-LFs were treated with MGF before and during mechanical stretch. We observed that OA delayed LFs response to mechanical injury, while MGF pretreatment promoted cells timely feedback the mechanically stimuli by inducing cellular stress. Additionally, MGF accelerated the ligament injury repair by promoting cell migration, decreasing the MMP-2 activity, and remitting the cell deformation via ATF-2 activating in cells. Conclusions: Our study shows that MGF pretreatment of OA-LFs can respond quickly to mechanical damage and repair ligament tissue by activating ATF-2. Therefore, MGF has potential as a therapeutic for OA patients.
KW - Activating transcription factor-2
KW - Ligament fibroblasts
KW - Mechanical injury
KW - Mechano growth factor
KW - Osteoarthritis
UR - https://www.scopus.com/pages/publications/85082433170
U2 - 10.1007/s10529-020-02866-5
DO - 10.1007/s10529-020-02866-5
M3 - 文章
C2 - 32222864
AN - SCOPUS:85082433170
SN - 0141-5492
VL - 42
SP - 1337
EP - 1349
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 8
ER -