TY - GEN
T1 - Label-free Paper-based Immunosensor with Graphene Nanocomposites for Electrochemical Detection of Follicle-stimulating Hormone
AU - Luo, Jinping
AU - Kong, Zhuang
AU - Wang, Yang
AU - Xie, Jingyu
AU - Liu, Juntao
AU - Jin, Hongyan
AU - Cai, Xinxia
N1 - Publisher Copyright:
© 2018 IEEE.
PY - 2018/10/26
Y1 - 2018/10/26
N2 - Follicle-stimulating hormone (FSH) is an important indicator of ovarian reserve function in women in clinical testing. In this work, a label-free paper-based immunosensor was developed for electrochemical rapid detection of FSH. A hydrophilic channel surrounded with hydrophobic barriers was firstly fabricated on the chromatography paper by wax printing technology. Then three electrodes were screen-printed on the circle zones of the channel, in which one carbon electrode further modified by reduced graphene-oxide /thionine /gold nanoparticles nanocomposites and FSH monoclonal antibody was used as the working electrode to provide sensitivity of the immunosensor. The detection of FSH is based on the decreased electrochemical current of Thi produced from the specific binding of the FSH and anti-FSH, and the decrease of the current is proportional to the concentration of the FSH. The experimental results exhibited that the immunosensor could be used to detect the standard FSH in range of 1-100 mIU/mL with the detection limit of 1 mIU/mL. And the proposed immunosensor had been successfully applied to detect FSH in serum samples.
AB - Follicle-stimulating hormone (FSH) is an important indicator of ovarian reserve function in women in clinical testing. In this work, a label-free paper-based immunosensor was developed for electrochemical rapid detection of FSH. A hydrophilic channel surrounded with hydrophobic barriers was firstly fabricated on the chromatography paper by wax printing technology. Then three electrodes were screen-printed on the circle zones of the channel, in which one carbon electrode further modified by reduced graphene-oxide /thionine /gold nanoparticles nanocomposites and FSH monoclonal antibody was used as the working electrode to provide sensitivity of the immunosensor. The detection of FSH is based on the decreased electrochemical current of Thi produced from the specific binding of the FSH and anti-FSH, and the decrease of the current is proportional to the concentration of the FSH. The experimental results exhibited that the immunosensor could be used to detect the standard FSH in range of 1-100 mIU/mL with the detection limit of 1 mIU/mL. And the proposed immunosensor had been successfully applied to detect FSH in serum samples.
UR - https://www.scopus.com/pages/publications/85056598137
U2 - 10.1109/EMBC.2018.8512923
DO - 10.1109/EMBC.2018.8512923
M3 - 会议稿件
C2 - 30441007
AN - SCOPUS:85056598137
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 2901
EP - 2904
BT - 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
Y2 - 18 July 2018 through 21 July 2018
ER -