Experimental study of moirá method in laser scanning confocal microscopy

By integrating moirá method with laser scanning confocal microscopy (LSCM), a novel class of moirá patterns, i.e., LSCM moirá patterns, is presented and investigated by experiment in this article. The moirá patterns are formed by the interference of a 1200 linemm cross-line holographic grating and the scanning lines of LSCM. The principles and conditions of forming LSCM moirá are described in detail. The measured parameters of moirá fringes from experimental images agree well with theoretical analysis. The experimental results verify the feasibility and reality of the proposed moirá method to be another effective and high accuracy technique for measuring and observing in-plane microdeformation at micrometer scale.