@inbook{c40ff8de6b914736ad3e841b66e7a4ee,
title = "Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-Function Studies of Target Gene Via Knockout",
abstract = "CRISPR-Cas9 is a unique technology that enables geneticists and medical researchers to edit genomic DNA for studying biology, pathogenesis, and molecular basis of treatment in malignant B cells. Unfortunately, malignant B cells are extremely difficult to transfect by most traditional methods. In this chapter, we describe the use of the Nucleofector{\texttrademark} Technology-based electroporation system with optimized transfection conditions for generating a malignant B cell model, JEKO-1, with ROR1-gene knockout via CRISPR-Cas9 technology.",
keywords = "CRISPR-Cas9, Electroporation, JEKO-1, Nucleofector",
author = "Jian Yu",
note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-4939-9740-4\_9",
language = "英语",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "85--90",
booktitle = "Methods in Molecular Biology",
}