TY - JOUR
T1 - EGFR antibody conjugated bimetallic Au@Ag nanorods for enhanced SERS-based tumor boundary identification, targeted photoacoustic imaging and photothermal therapy
AU - Chen, Shouhui
AU - Bao, Chenchen
AU - Zhang, Chunlei
AU - Yang, Yao
AU - Wang, Kan
AU - Chikkaveeraiah, Bhaskara V.
AU - Wang, Zhihua
AU - Huang, Xinglu
AU - Pan, Fei
AU - Wang, Kun
AU - Zhi, Xiao
AU - Ni, Jian
AU - de la Fuente, Jesus M.
AU - Tian, Jie
N1 - Publisher Copyright:
© 2016 Shouhui Chen, Chenchen Bao, Chunlei Zhang, Yao Yang, Kan Wang, Bhaskara V. Chikkaveeraiah, Zhihua Wang, Xinglu Huang, Fei Pan, Kun Wang, Xiao Zhi, Jian Ni, Jesus M. de la Fuente, and Jie Tian.
PY - 2016
Y1 - 2016
N2 - The development of multifunctional nanoprobes for simultaneous targeted imaging, tumor boundary identification and photothermal therapy of gastric cancer has become a great challenge. Herein, EGFR monoclonal antibody-conjugated Au@Ag nanorods decorated with DTNB nanoprobes (5,5'-Dithiobis-(2-nitrobenzoic acid)) (EGFR-Au@Ag NR nanotags) were prepared and characterized. Their biocompatibility was analyzed by MTT assay. In vitro studies show that EGFR-Au@Ag NR nanotags own good biocompatibility and capability of targeting and entering into gastric cancer MGC803 cells, silver nano-film layer on the surface of gold nanorods remarkably enhance surfaceenhanced rama spectra (SERS) signal, enhanced photoacoustic imaging efficacy and photothermal conversion efficiency of gold nanorods. In vivo studies show that prepared nanotags could target actively gastric cancer cells at 2 h post-injection, and distributed in the tumor site, exhibited enhanced SERS signals to display clearly tumor boundary, enhanced photoacoustic imaging to display clearly tumor boundary. Under 1 w/cm2 laser irradiation, tumor growth was remarkably inhibited by local photothermal therapy. In conclusion, high performance EGFR-antibody conjugated Au@Ag nanorod- DTNB nanoprobes exhibit great potential in applications such as targeted photoacoustic imaging, simultaneous tumor boundary identification and selective photothermal therapy of gastric cancer in the near future.
AB - The development of multifunctional nanoprobes for simultaneous targeted imaging, tumor boundary identification and photothermal therapy of gastric cancer has become a great challenge. Herein, EGFR monoclonal antibody-conjugated Au@Ag nanorods decorated with DTNB nanoprobes (5,5'-Dithiobis-(2-nitrobenzoic acid)) (EGFR-Au@Ag NR nanotags) were prepared and characterized. Their biocompatibility was analyzed by MTT assay. In vitro studies show that EGFR-Au@Ag NR nanotags own good biocompatibility and capability of targeting and entering into gastric cancer MGC803 cells, silver nano-film layer on the surface of gold nanorods remarkably enhance surfaceenhanced rama spectra (SERS) signal, enhanced photoacoustic imaging efficacy and photothermal conversion efficiency of gold nanorods. In vivo studies show that prepared nanotags could target actively gastric cancer cells at 2 h post-injection, and distributed in the tumor site, exhibited enhanced SERS signals to display clearly tumor boundary, enhanced photoacoustic imaging to display clearly tumor boundary. Under 1 w/cm2 laser irradiation, tumor growth was remarkably inhibited by local photothermal therapy. In conclusion, high performance EGFR-antibody conjugated Au@Ag nanorod- DTNB nanoprobes exhibit great potential in applications such as targeted photoacoustic imaging, simultaneous tumor boundary identification and selective photothermal therapy of gastric cancer in the near future.
KW - Au@Ag nanorods
KW - Gastric cancer
KW - Photoacoustic imaging
KW - Photothermal therapy
KW - SERS imaging
KW - Tumor boundary
UR - https://www.scopus.com/pages/publications/85010650951
U2 - 10.5101/nbe.v8i4.p315-328
DO - 10.5101/nbe.v8i4.p315-328
M3 - 文章
AN - SCOPUS:85010650951
SN - 2150-5578
VL - 8
SP - 315
EP - 328
JO - Nano Biomedicine and Engineering
JF - Nano Biomedicine and Engineering
IS - 4
ER -
可持续发展目标 3 良好健康与福祉