Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

Qi Zhang; Yan Xiang; Dawei Liang; Yinggui Peng; Hong Guo

doi:10.1016/j.bmcl.2011.11.028

Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

Qi Zhang
, Yan Xiang^*
, Dawei Liang
, Yinggui Peng
, Hong Guo

^*此作品的通讯作者

Beihang University

科研成果: 期刊稿件 › 文章 › 同行评审

4 引用（Scopus）

摘要

N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (k_obs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 10⁷-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.

源语言	英语
页（从-至）	1814-1817
页数	4
期刊	Bioorganic and Medicinal Chemistry Letters
卷	22
期	4
DOI	https://doi.org/10.1016/j.bmcl.2011.11.028
出版状态	已出版 - 15 2月 2012

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title = "Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex",

abstract = "N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (kobs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 107-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.",

keywords = "Artificial nuclease, Chitosan, DNA cleavage, Double-strand, Zinc complex",

author = "Qi Zhang and Yan Xiang and Dawei Liang and Yinggui Peng and Hong Guo",

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doi = "10.1016/j.bmcl.2011.11.028",

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TY - JOUR

T1 - Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

AU - Zhang, Qi

AU - Xiang, Yan

AU - Liang, Dawei

AU - Peng, Yinggui

AU - Guo, Hong

PY - 2012/2/15

Y1 - 2012/2/15

N2 - N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (kobs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 107-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.

AB - N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (kobs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 107-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.

KW - Artificial nuclease

KW - Chitosan

KW - DNA cleavage

KW - Double-strand

KW - Zinc complex

UR - https://www.scopus.com/pages/publications/84862793026

U2 - 10.1016/j.bmcl.2011.11.028

DO - 10.1016/j.bmcl.2011.11.028

M3 - 文章

AN - SCOPUS:84862793026

SN - 0960-894X

VL - 22

SP - 1814

EP - 1817

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 4

ER -

Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

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