TY - JOUR
T1 - Effect of simulated transport stress on the rat small intestine
T2 - A morphological and gene expression study
AU - Wan, Changrong
AU - Yin, Peng
AU - Xu, Xiaolong
AU - Liu, Mingjiang
AU - He, Shasha
AU - Song, Shixiu
AU - Liu, Fenghua
AU - Xu, Jianqin
PY - 2014/4
Y1 - 2014/4
N2 - The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35. °C and 0.1×. g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P<. 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P<. 0.01), as well as expression of Hsp27/70/90 mRNA (P<. 0.05; P<. 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine.
AB - The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35. °C and 0.1×. g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P<. 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P<. 0.01), as well as expression of Hsp27/70/90 mRNA (P<. 0.05; P<. 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine.
KW - Intestinal damage
KW - Microarray analysis
KW - Morphological study
KW - Transport stress
UR - https://www.scopus.com/pages/publications/84896491137
U2 - 10.1016/j.rvsc.2014.01.008
DO - 10.1016/j.rvsc.2014.01.008
M3 - 文章
C2 - 24560020
AN - SCOPUS:84896491137
SN - 0034-5288
VL - 96
SP - 355
EP - 364
JO - Research in Veterinary Science
JF - Research in Veterinary Science
IS - 2
ER -