TY - GEN
T1 - Comparative study on biological effects of two-dimensional and three-dimensional simulated microgravity
AU - Sun, Lianwen
AU - Fan, Yubo
AU - Xie, Tian
AU - Gan, Bo
AU - Hu, Qinghua
PY - 2007
Y1 - 2007
N2 - Microgravity (MG) is the most important characteristic in space flight, which affects the endocrine, metabolic, immune and musculoskeletal systems. Because of the limitations of space experiment, the ground-based experiments are very important to understanding the physiological and pathophysiological changes in space flight. To simulate microgravity on ground there are two methods for adherent cells. One is three-dimensional MG using a rotary cell culture system (RCCS) equipped with high aspect ratio vessels. The other one is two-dimensional MG using cell roller on which cell dish was put. Both can produce altered-vector of gravity that mimics gravity. In this study, the effects of two-dimensional (2D) and three-dimensional (3D) MG on cell proliferation and differentiation were determined. The results showed that similar biological effects on cells under both MG models, which are inhibition of cell proliferation, alkaline phosphatase (ALP) activity in MC3T3-E1 and telomerase activity in mesenchymal stem cells (MSCs). Considering easily control setting and simple manipulation, we prefer 2D simulated microgravity as ground-based model.
AB - Microgravity (MG) is the most important characteristic in space flight, which affects the endocrine, metabolic, immune and musculoskeletal systems. Because of the limitations of space experiment, the ground-based experiments are very important to understanding the physiological and pathophysiological changes in space flight. To simulate microgravity on ground there are two methods for adherent cells. One is three-dimensional MG using a rotary cell culture system (RCCS) equipped with high aspect ratio vessels. The other one is two-dimensional MG using cell roller on which cell dish was put. Both can produce altered-vector of gravity that mimics gravity. In this study, the effects of two-dimensional (2D) and three-dimensional (3D) MG on cell proliferation and differentiation were determined. The results showed that similar biological effects on cells under both MG models, which are inhibition of cell proliferation, alkaline phosphatase (ALP) activity in MC3T3-E1 and telomerase activity in mesenchymal stem cells (MSCs). Considering easily control setting and simple manipulation, we prefer 2D simulated microgravity as ground-based model.
KW - Biological effect
KW - Simulated microgravity
KW - Three dimensio
KW - Two dimension
UR - https://www.scopus.com/pages/publications/35348886151
U2 - 10.1109/ICBBE.2007.124
DO - 10.1109/ICBBE.2007.124
M3 - 会议稿件
AN - SCOPUS:35348886151
SN - 1424411203
SN - 9781424411207
T3 - 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE
SP - 470
EP - 473
BT - 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE
T2 - 2007 1st International Conference on Bioinformatics and Biomedical Engineering, ICBBE
Y2 - 6 July 2007 through 8 July 2007
ER -