Bioinformatic analysis of cardiac and pulmonary hypoxia

BRB-ArrayTools was used to analyze differentially expressed genes of samples of pulmonary microvascular endothelial cells and cardiac microvascular endothelial cells cultured in hypoxia. 39 and 66 differentially expressed genes were obtained from pulmonary cells and cardiac cells respectively. The differentially expressed genes showed a relatively stable expression trend in response to hypoxia with the increase of hypoxia time. Genecodis analisis showed that the differentially expressed genes were involved in angiogenesis, NAD+activity, transmembrane transport, redox processes, and oxidative stress. Literature mining was applied to obtain the hypoxia-regulated microRNAs (miRNAs) from previously published literature. The hypoxia-regulated miRNAs were involved in VEGF (vascular endothelial growth-factor) receptor function, Wnt and MAPK (mitogen-activated protein kinase) pathway. The results show that mining bioinformation in gene chip and integrating distributed resources of the literature database could deepen understanding of biological processes of genes and miRNAs involved in hypoxia, and these data benefit further investigation and cognitive perspective of the molecular mechanisms of hypoxia.