A Self-Assembled DNA Origami-Gold Nanorod Complex for Cancer Theranostics

A self-assembled DNA origami (DO)-gold nanorod (GNR) complex, which is a dual-functional nanotheranostics constructed by decorating GNRs onto the surface of DNA origami, is demonstrated. After 24 h incubation of two structured DO-GNR complexes with human MCF7 breast cancer cells, significant enhancement of cell uptake is achieved compared to bare GNRs by two-photon luminescence imaging. Particularly, the triangle shaped DO-GNR complex exhibits optimal cellular accumulation. Compared to GNRs, improved photothermolysis against tumor cells is accomplished for the triangle DO-GNR complex by two-photon laser or NIR laser irradiation. Moreover, the DO-GNR complex exhibits enhanced antitumor efficacy compared with bare GNRs in nude mice bearing breast tumor xenografts. The results demonstrate that the DO-GNR complex can achieve optimal two-photon cell imaging and photothermal effect, suggesting a promising candidate for cancer diagnosis and therapy both in vitro and in vivo. To integrate photothermal ablation with effective drug delivery for improved cancer therapy, a dual-functional nanotheranostics is constructed by assembling gold nanorods (GNR) on a DNA origami (DO) platform. Compared to GNRs, improved photoluminescence-imaging and photothermolysis are accomplished for DO-GNR against tumor cells and tumor bearing mice. The hybrid nanostructures provide a promising candidate for cancer diagnosis and therapy.