Unsupervised Histological Image Registration Using Structural Feature Guided Convolutional Neural Network

Registration of multiple stained images is a fundamental task in histological image analysis. In supervised methods, obtaining ground-truth data with known correspondences is laborious and time-consuming. Thus, unsupervised methods are expected. Unsupervised methods ease the burden of manual annotation but often at the cost of inferior results. In addition, registration of histological images suffers from appearance variance due to multiple staining, repetitive texture, and section missing during making tissue sections. To deal with these challenges, we propose an unsupervised structural feature guided convolutional neural network (SFG). Structural features are robust to multiple staining. The combination of low-resolution rough structural features and high-resolution fine structural features can overcome repetitive texture and section missing, respectively. SFG consists of two components of structural consistency constraints according to the formations of structural features, i.e., dense structural component and sparse structural component. The dense structural component uses structural feature maps of the whole image as structural consistency constraints, which represent local contextual information. The sparse structural component utilizes the distance of automatically obtained matched key points as structural consistency constraints because the matched key points in an image pair emphasize the matching of significant structures, which imply global information. In addition, a multi-scale strategy is used in both dense and sparse structural components to make full use of the structural information at low resolution and high resolution to overcome repetitive texture and section missing. The proposed method was evaluated on a public histological dataset (ANHIR) and ranked first as of Jan 18th, 2022.