Two-photon laser scanning stereomicroscopy for fast volumetric imaging

Yanlong Yang; Baoli Yao; Ming Lei; Dan Dan; Runze Li; Mark Van Horn; Xun Chen; Yang Li; Tong Ye

doi:10.1371/journal.pone.0168885

Two-photon laser scanning stereomicroscopy for fast volumetric imaging

Yanlong Yang
, Baoli Yao
, Ming Lei
, Dan Dan
, Runze Li
, Mark Van Horn
, Xun Chen
, Yang Li
, Tong Ye

CAS - Xi'an Institute of Optics and Precision Mechanics
Medical University of South Carolina
Clemson University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

Original language	English
Article number	e0168885
Journal	PLOS ONE
Volume	11
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0168885
State	Published - Dec 2016
Externally published	Yes

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abstract = "Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.",

author = "Yanlong Yang and Baoli Yao and Ming Lei and Dan Dan and Runze Li and \{Van Horn\}, Mark and Xun Chen and Yang Li and Tong Ye",

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AU - Yang, Yanlong

AU - Yao, Baoli

AU - Lei, Ming

AU - Dan, Dan

AU - Li, Runze

AU - Van Horn, Mark

AU - Chen, Xun

AU - Li, Yang

AU - Ye, Tong

N1 - Publisher Copyright: © This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

PY - 2016/12

Y1 - 2016/12

N2 - Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

AB - Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

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Two-photon laser scanning stereomicroscopy for fast volumetric imaging

Abstract

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