The selection and characterization of the mutants in bioflocculant bacteria C8 induced by 3 MeV helium ion irradiation

L. L. Xu; Y. N. Yang; J. M. Xue; X. L. Ding; R. Q. Guo

doi:10.1016/j.nimb.2008.11.007

The selection and characterization of the mutants in bioflocculant bacteria C8 induced by 3 MeV helium ion irradiation

L. L. Xu
, Y. N. Yang^*
, J. M. Xue
, X. L. Ding
, R. Q. Guo

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A 3 MeV He²⁺ beam was used to irradiate C8 (a flocculant-producing bacteria) with a fluence ranging from 10¹¹ to 10¹³ ions/cm². The effects on the survival ratio, TTC-dehydrogenase activity, flocculating activity and RAPD analysis are reported. The survival ratio curve caused by irradiation is proved to be "saddle-shaped". Eleven mutants were obtained, all of which had a significant change in dehydrogenase activity and most showed a positive change in flocculating activity. RAPD measurements were used to analyse the DNA of mutants with a flocculating activity over 80%, which indicated that all their DNA had been changed by irradiation.

Original language	English
Pages (from-to)	99-102
Number of pages	4
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	267
Issue number	1
DOIs	https://doi.org/10.1016/j.nimb.2008.11.007
State	Published - Jan 2009

Keywords

Bioflocculant
Dehydrogenase
Heavy ions
Ion irradiation
RAPD
Survival ratio

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10.1016/j.nimb.2008.11.007

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title = "The selection and characterization of the mutants in bioflocculant bacteria C8 induced by 3 MeV helium ion irradiation",

abstract = "A 3 MeV He2+ beam was used to irradiate C8 (a flocculant-producing bacteria) with a fluence ranging from 1011 to 1013 ions/cm2. The effects on the survival ratio, TTC-dehydrogenase activity, flocculating activity and RAPD analysis are reported. The survival ratio curve caused by irradiation is proved to be {"}saddle-shaped{"}. Eleven mutants were obtained, all of which had a significant change in dehydrogenase activity and most showed a positive change in flocculating activity. RAPD measurements were used to analyse the DNA of mutants with a flocculating activity over 80\%, which indicated that all their DNA had been changed by irradiation.",

keywords = "Bioflocculant, Dehydrogenase, Heavy ions, Ion irradiation, RAPD, Survival ratio",

author = "Xu, \{L. L.\} and Yang, \{Y. N.\} and Xue, \{J. M.\} and Ding, \{X. L.\} and Guo, \{R. Q.\}",

year = "2009",

month = jan,

doi = "10.1016/j.nimb.2008.11.007",

language = "英语",

volume = "267",

pages = "99--102",

journal = "Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms",

issn = "0168-583X",

publisher = "Elsevier B.V.",

number = "1",

}

The selection and characterization of the mutants in bioflocculant bacteria C8 induced by 3 MeV helium ion irradiation. / Xu, L. L.; Yang, Y. N.; Xue, J. M. et al.
In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 267, No. 1, 01.2009, p. 99-102.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The selection and characterization of the mutants in bioflocculant bacteria C8 induced by 3 MeV helium ion irradiation

AU - Xu, L. L.

AU - Yang, Y. N.

AU - Xue, J. M.

AU - Ding, X. L.

AU - Guo, R. Q.

PY - 2009/1

Y1 - 2009/1

N2 - A 3 MeV He2+ beam was used to irradiate C8 (a flocculant-producing bacteria) with a fluence ranging from 1011 to 1013 ions/cm2. The effects on the survival ratio, TTC-dehydrogenase activity, flocculating activity and RAPD analysis are reported. The survival ratio curve caused by irradiation is proved to be "saddle-shaped". Eleven mutants were obtained, all of which had a significant change in dehydrogenase activity and most showed a positive change in flocculating activity. RAPD measurements were used to analyse the DNA of mutants with a flocculating activity over 80%, which indicated that all their DNA had been changed by irradiation.

AB - A 3 MeV He2+ beam was used to irradiate C8 (a flocculant-producing bacteria) with a fluence ranging from 1011 to 1013 ions/cm2. The effects on the survival ratio, TTC-dehydrogenase activity, flocculating activity and RAPD analysis are reported. The survival ratio curve caused by irradiation is proved to be "saddle-shaped". Eleven mutants were obtained, all of which had a significant change in dehydrogenase activity and most showed a positive change in flocculating activity. RAPD measurements were used to analyse the DNA of mutants with a flocculating activity over 80%, which indicated that all their DNA had been changed by irradiation.

KW - Bioflocculant

KW - Dehydrogenase

KW - Heavy ions

KW - Ion irradiation

KW - RAPD

KW - Survival ratio

UR - https://www.scopus.com/pages/publications/58749100279

U2 - 10.1016/j.nimb.2008.11.007

DO - 10.1016/j.nimb.2008.11.007

M3 - 文章

AN - SCOPUS:58749100279

SN - 0168-583X

VL - 267

SP - 99

EP - 102

JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

IS - 1

ER -

The selection and characterization of the mutants in bioflocculant bacteria C8 induced by 3 MeV helium ion irradiation

Abstract

Keywords

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