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The observation of the PG-depletion in progressive trypsin digested articular cartilage based on conventional a-mode ultrasound

  • Beihang University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This study tried to monitor proteoglycans (PG)-depletion in progressive trypsin digested porcine articular cartilage (AC) samples with a conventional ultrasound system. 10 cartilage-bone samples were prepared from normal porcine femoral condyle cartilages and were digested with 0.25% trypsin solution for two hours. The dynamic process of the PG digestion was explored using an A-mode ultrasound experiment system with 10MHz center frequency. The speed of PG-depletion and the degree of PG-depletion were estimated from ultrasound echo signal. The results showed that 10MHz ultrasound is valuable in tracking the degree of PG-digestion. The transient speed of PG-digestion decreased with digesting time and tissue depth. The digestion speed is faster in the superficial layer of the cartilage samples (0.93 ± 0.3M/min) than that in deeper layers (0.26 ± 0.12 M/min at 50% of the full thickness). The histological image also confirmed the validity of the ultrasound observation.. This study may provide some useful information in identifying cartilage matrix integrity and osteoarthritis (OA) disease progression.

Original languageEnglish
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Pages1101-1104
Number of pages4
DOIs
StatePublished - 2013
EventWorld Congress on Medical Physics and Biomedical Engineering - Beijing, China
Duration: 26 May 201231 May 2012

Publication series

NameIFMBE Proceedings
Volume39 IFMBE
ISSN (Print)1680-0737

Conference

ConferenceWorld Congress on Medical Physics and Biomedical Engineering
Country/TerritoryChina
CityBeijing
Period26/05/1231/05/12

Keywords

  • Articular cartilage
  • Conventional Ultrasound
  • Osteoarthritis
  • PG-depletion
  • Trypsin degeneration

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