Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

Zhongtao Hu; Yaoheng Yang; Dezhuang Ye; Si Chen; Yan Gong; Chinwendu Chukwu; Hong Chen

doi:10.1016/j.xpro.2023.102132

Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

Zhongtao Hu^*
, Yaoheng Yang
, Dezhuang Ye
, Si Chen
, Yan Gong
, Chinwendu Chukwu
, Hong Chen^*

^*Corresponding author for this work

Washington University St. Louis

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).¹

Original language	English
Article number	102132
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2023.102132
State	Published - 17 Mar 2023
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biotechnology and bioengineering
Cancer
Health Sciences
Neuroscience
Physics

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10.1016/j.xpro.2023.102132

Cite this

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title = "Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device",

abstract = "Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).1",

keywords = "Biotechnology and bioengineering, Cancer, Health Sciences, Neuroscience, Physics",

author = "Zhongtao Hu and Yaoheng Yang and Dezhuang Ye and Si Chen and Yan Gong and Chinwendu Chukwu and Hong Chen",

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year = "2023",

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doi = "10.1016/j.xpro.2023.102132",

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AU - Hu, Zhongtao

AU - Yang, Yaoheng

AU - Ye, Dezhuang

AU - Chen, Si

AU - Gong, Yan

AU - Chukwu, Chinwendu

AU - Chen, Hong

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).1

AB - Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).1

KW - Biotechnology and bioengineering

KW - Cancer

KW - Health Sciences

KW - Neuroscience

KW - Physics

UR - https://www.scopus.com/pages/publications/85148753500

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DO - 10.1016/j.xpro.2023.102132

M3 - 文章

C2 - 36861835

AN - SCOPUS:85148753500

SN - 2666-1667

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JO - STAR Protocols

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M1 - 102132

ER -

Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

Abstract

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Keywords

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