Synthesis and characterization of RGDS- and collagen-modified poly(DL-lactic acid)

Maleic anhydride (MAH), butanediamine (BDA) and RGDS adhesive peptide or collagen type I were introduced in turn into poly(DL-lactic acid) (PDLLA) to give novel biomimetic polymers: collagen-modified PDLLA (CPLA) and RGDS-modified PDLLA (RGDS-PLA). FT-IR, GPC-MALLS, Rhodamine-carboxyl interaction method and ninhydrin reaction were employed to characterize MAH-modified PDLLA (MPLA) and BDA-modified PDLLA (BDPLA); FITC fluorescence labeling method was used to qualitatively characterize CPLA and amino acid analyzer to determine the content of RGDS in RGDS-PLA. The results reveal that those extracellular matrix proteins such as collagen and RGDS can be incorporated into the side chain of PDLLA by means of the developed synthesis method, producing a new family of PDLLA-based biomimetic materials.