Study on binding characteristics of (S)-naproxen imprinted polymer and performance of its complexes during preparation

A molecularly imprinted polymer (MIP) was synthesized as the highly chiral selective materials for (S)-naproxen with acrylamide as functional monomers. UV (ultra-violet) studies showed that the template (S)-naproxen and functional monomer acrylamide formed complexes before polymerization and Hyperchem was used to simulate the structures of complexes. The binding capacity of MIP to (S)-naproxen is higher than that of its enantiomer and the chiral separation factor α is 1.87. Scatchard analysis suggests the MIP recognizing (S)-naproxen with two classes of binding sites. The calculated dissociation constant K_d,1 and apparent maximum number Q_max,1 of binding sites with high affinity are 28.0 μmol/L and 52.94 μmol/g respectively, while K_d,2 and Q_max,2 of binding sites with low affinity are 0.489 mmol/L and 0.122 mmol/g.