Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber

Conghao Wang; Huilan Liu; Jianrui Ma; Haodong Cui; Yijun Li; Danlei Wu; Yanhui Hu; Dakun Wu; Qiang Fu; Lintang Liang; Fei Yu; Runlong Wu; Aimin Wang; Lishuang Feng

doi:10.1364/OE.444720

Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber

Conghao Wang
, Huilan Liu
, Jianrui Ma
, Haodong Cui
, Yijun Li
, Danlei Wu
, Yanhui Hu
, Dakun Wu
, Qiang Fu
, Lintang Liang
, Fei Yu
, Runlong Wu
, Aimin Wang
, Lishuang Feng^*

^*Corresponding author for this work

School of Instrumentation and Optoelectronic Engineering

Beihang University
Key Laboratory of Precision Opto-Mechatronics Technology (Ministry of Education)
Ltd
University of Chinese Academy of Sciences
CAS - Shanghai Institute of Optics and Fine Mechanics
Peking University

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Two-photon endomicroscopy is an optical biopsy tool that satisfies clinical requirements for real-time subcellular-resolution imaging to assist pathological biopsy in diagnosis. Herein, we present a two-photon endomicroscopy system based on a piezoelectric ceramic tube scanner. A dual-channel amplitude-modulated sine wave drives the fiber to realize spiral scanning, a double-cladding antiresonant fiber is used for 920-nm femtosecond light-pulse low dispersion transmission, and fluorescence collection occurs with no fiber-tip engineering. The field of view is ∼200 µm, the resolution is 3.1 µm, and the frame rate is 0.7 fps. Pollen grain, GFP-labeled mouse brain section, and human stomach tissue imaging verify the capability of the two-photon endomicroscopy system.

Original language	English
Pages (from-to)	43124-43135
Number of pages	12
Journal	Optics Express
Volume	29
Issue number	26
DOIs	https://doi.org/10.1364/OE.444720
State	Published - 20 Dec 2021

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10.1364/OE.444720

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title = "Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber",

abstract = "Two-photon endomicroscopy is an optical biopsy tool that satisfies clinical requirements for real-time subcellular-resolution imaging to assist pathological biopsy in diagnosis. Herein, we present a two-photon endomicroscopy system based on a piezoelectric ceramic tube scanner. A dual-channel amplitude-modulated sine wave drives the fiber to realize spiral scanning, a double-cladding antiresonant fiber is used for 920-nm femtosecond light-pulse low dispersion transmission, and fluorescence collection occurs with no fiber-tip engineering. The field of view is ∼200 µm, the resolution is 3.1 µm, and the frame rate is 0.7 fps. Pollen grain, GFP-labeled mouse brain section, and human stomach tissue imaging verify the capability of the two-photon endomicroscopy system.",

author = "Conghao Wang and Huilan Liu and Jianrui Ma and Haodong Cui and Yijun Li and Danlei Wu and Yanhui Hu and Dakun Wu and Qiang Fu and Lintang Liang and Fei Yu and Runlong Wu and Aimin Wang and Lishuang Feng",

note = "Publisher Copyright: {\textcopyright} 2021 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement",

year = "2021",

month = dec,

day = "20",

doi = "10.1364/OE.444720",

language = "英语",

volume = "29",

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publisher = "Optica Publishing Group (formerly OSA)",

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TY - JOUR

T1 - Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber

AU - Wang, Conghao

AU - Liu, Huilan

AU - Ma, Jianrui

AU - Cui, Haodong

AU - Li, Yijun

AU - Wu, Danlei

AU - Hu, Yanhui

AU - Wu, Dakun

AU - Fu, Qiang

AU - Liang, Lintang

AU - Yu, Fei

AU - Wu, Runlong

AU - Wang, Aimin

AU - Feng, Lishuang

PY - 2021/12/20

Y1 - 2021/12/20

N2 - Two-photon endomicroscopy is an optical biopsy tool that satisfies clinical requirements for real-time subcellular-resolution imaging to assist pathological biopsy in diagnosis. Herein, we present a two-photon endomicroscopy system based on a piezoelectric ceramic tube scanner. A dual-channel amplitude-modulated sine wave drives the fiber to realize spiral scanning, a double-cladding antiresonant fiber is used for 920-nm femtosecond light-pulse low dispersion transmission, and fluorescence collection occurs with no fiber-tip engineering. The field of view is ∼200 µm, the resolution is 3.1 µm, and the frame rate is 0.7 fps. Pollen grain, GFP-labeled mouse brain section, and human stomach tissue imaging verify the capability of the two-photon endomicroscopy system.

AB - Two-photon endomicroscopy is an optical biopsy tool that satisfies clinical requirements for real-time subcellular-resolution imaging to assist pathological biopsy in diagnosis. Herein, we present a two-photon endomicroscopy system based on a piezoelectric ceramic tube scanner. A dual-channel amplitude-modulated sine wave drives the fiber to realize spiral scanning, a double-cladding antiresonant fiber is used for 920-nm femtosecond light-pulse low dispersion transmission, and fluorescence collection occurs with no fiber-tip engineering. The field of view is ∼200 µm, the resolution is 3.1 µm, and the frame rate is 0.7 fps. Pollen grain, GFP-labeled mouse brain section, and human stomach tissue imaging verify the capability of the two-photon endomicroscopy system.

UR - https://www.scopus.com/pages/publications/85121117122

U2 - 10.1364/OE.444720

DO - 10.1364/OE.444720

M3 - 文章

AN - SCOPUS:85121117122

SN - 1094-4087

VL - 29

SP - 43124

EP - 43135

JO - Optics Express

JF - Optics Express

IS - 26

ER -

Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber

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