Small-volume solution current-time behavior study for application in reverse iontophoresis-based non-invasive blood glucose monitoring

The reverse iontophoresis-based glucose monitoring circumstance is similar to the small-volume solution in which mass diffusion controls the current response of the electrochemical biosensors. In this study, the law of mass transfer in this type of solution was analyzed and a mathematic model was established to depict the current-time behavior of the fabricated planar electrode used in the non-invasive meter designed by ourselves. A small-volume glucose solution was directly constructed on the electrode to simulate the reverse iontophoresis-based sensing condition. The correctness of the model was demonstrated by chronoamperometry. Animal assay was subsequently carried out to verify the practicality of the model in determination of blood glucose. The results processed by the new method accurately traced the authentic value, confirming the advantage of the new method and the potential in clinical analysis.