Series microscopy images fusion based on area wavelet transform

Digital image fusion technique based on multi-focus series microscopy images is an effective method to extend the depth of focus. In order to avoid the blocking effect of fused image by spatial domain fusion algorithm when large focusing step is chosen or the image samples are transparent, a series microscopy images fusion algorithm based on area wavelet transform is proposed. Considering the fact that neighboring pixels are related and wavelet transform has the multi-resolution merit, fusion strategies are as follow: fusion operators are different corresponding to different frequency region, and fusion process is based on region coefficients of wavelet transform. Meanwhile, the influences of different wavelet bases on performance of fusion are investigated. Experimental results show that the blur is improved in obtained fused image and the blocking effect is eliminated. The fusion performance of this method is satisfactory.