SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM

Zaizai Dong; Lingqian Chang

SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM

Zaizai Dong
, Lingqian Chang^*

^*Corresponding author for this work

School of Engineering Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Enhancers involve in the upregulation of multiple oncogenes, playing a fundamental role in tumorigenesis. We developed a nanoelectroporation-probing (NP) platform that enables sensitive interrogation of enhancer activity in living cells. The nanoelectroporation biochip achieved highly-focused perforation of cell membrane and expedited the delivery of enhancer probes into nucleus. The enhancer probes (named ‘PH probe') increased the fluorescence signal over 10-fold within 1 hour. This platform was leveraged to distinguish the increased activity of CCAT1 enhancers in clinical cell samples, and reveal the existence of enhancer heterogeneity among lung cancer patients, providing a promising toolbox for intranuclear study of living cells.

Original language	English
Title of host publication	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1117-1118
Number of pages	2
ISBN (Electronic)	9781733419048
State	Published - 2022
Event	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: 23 Oct 2022 → 27 Oct 2022

Publication series

Name	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/Territory	China
City	Hybrid, Hangzhou
Period	23/10/22 → 27/10/22

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

DNA probe
biochip
enhancer
intracellular analysis
nanoelectroporation

Cite this

Dong, Z., & Chang, L. (2022). SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1117-1118). (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Dong, Zaizai ; Chang, Lingqian. / SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. pp. 1117-1118 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM",

abstract = "Enhancers involve in the upregulation of multiple oncogenes, playing a fundamental role in tumorigenesis. We developed a nanoelectroporation-probing (NP) platform that enables sensitive interrogation of enhancer activity in living cells. The nanoelectroporation biochip achieved highly-focused perforation of cell membrane and expedited the delivery of enhancer probes into nucleus. The enhancer probes (named {\textquoteleft}PH probe') increased the fluorescence signal over 10-fold within 1 hour. This platform was leveraged to distinguish the increased activity of CCAT1 enhancers in clinical cell samples, and reveal the existence of enhancer heterogeneity among lung cancer patients, providing a promising toolbox for intranuclear study of living cells.",

keywords = "DNA probe, biochip, enhancer, intracellular analysis, nanoelectroporation",

author = "Zaizai Dong and Lingqian Chang",

note = "Publisher Copyright: {\textcopyright} 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",

year = "2022",

language = "英语",

series = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1117--1118",

booktitle = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Dong, Z & Chang, L 2022, SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM. in MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1117-1118, 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022, Hybrid, Hangzhou, China, 23/10/22.

SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM. / Dong, Zaizai ; Chang, Lingqian.
MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. p. 1117-1118 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM

AU - Dong, Zaizai

AU - Chang, Lingqian

PY - 2022

Y1 - 2022

N2 - Enhancers involve in the upregulation of multiple oncogenes, playing a fundamental role in tumorigenesis. We developed a nanoelectroporation-probing (NP) platform that enables sensitive interrogation of enhancer activity in living cells. The nanoelectroporation biochip achieved highly-focused perforation of cell membrane and expedited the delivery of enhancer probes into nucleus. The enhancer probes (named ‘PH probe') increased the fluorescence signal over 10-fold within 1 hour. This platform was leveraged to distinguish the increased activity of CCAT1 enhancers in clinical cell samples, and reveal the existence of enhancer heterogeneity among lung cancer patients, providing a promising toolbox for intranuclear study of living cells.

AB - Enhancers involve in the upregulation of multiple oncogenes, playing a fundamental role in tumorigenesis. We developed a nanoelectroporation-probing (NP) platform that enables sensitive interrogation of enhancer activity in living cells. The nanoelectroporation biochip achieved highly-focused perforation of cell membrane and expedited the delivery of enhancer probes into nucleus. The enhancer probes (named ‘PH probe') increased the fluorescence signal over 10-fold within 1 hour. This platform was leveraged to distinguish the increased activity of CCAT1 enhancers in clinical cell samples, and reveal the existence of enhancer heterogeneity among lung cancer patients, providing a promising toolbox for intranuclear study of living cells.

KW - DNA probe

KW - biochip

KW - enhancer

KW - intracellular analysis

KW - nanoelectroporation

UR - https://www.scopus.com/pages/publications/85175704271

M3 - 会议稿件

AN - SCOPUS:85175704271

T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1117

EP - 1118

BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

Y2 - 23 October 2022 through 27 October 2022

ER -

Dong Z , Chang L. SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2022. p. 1117-1118. (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

SENSITIVE INTERROGATION OF ENHANCER ACTIVITY IN LIVING CELLS ON NANOELECTROPORATION-PROBING PLATFORM

Abstract

Publication series

Conference

UN SDGs

Keywords

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