Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei; Dorin Simionesie; Jan Sefcik; Jens U. Sutter; Qingjiang Xue; Jun Yu; Jinliang Wang; David J.S. Birch; Yu Chen

doi:10.1364/OL.40.005738

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei
, Dorin Simionesie
, Jan Sefcik
, Jens U. Sutter
, Qingjiang Xue
, Jun Yu
, Jinliang Wang
, David J.S. Birch
, Yu Chen^*

^*Corresponding author for this work

School of Physics

Beihang University
University of Strathclyde

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modeling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for realtime monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

Original language	English
Pages (from-to)	5738-5741
Number of pages	4
Journal	Optics Letters
Volume	40
Issue number	24
DOIs	https://doi.org/10.1364/OL.40.005738
State	Published - 15 Dec 2015

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title = "Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy",

abstract = "We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modeling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for realtime monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60\% increase in decay time component and 300\% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.",

author = "Guoke Wei and Dorin Simionesie and Jan Sefcik and Sutter, \{Jens U.\} and Qingjiang Xue and Jun Yu and Jinliang Wang and Birch, \{David J.S.\} and Yu Chen",

note = "Publisher Copyright: {\textcopyright} 2015 Optical Society of America.",

year = "2015",

month = dec,

day = "15",

doi = "10.1364/OL.40.005738",

language = "英语",

volume = "40",

pages = "5738--5741",

journal = "Optics Letters",

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TY - JOUR

T1 - Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

AU - Wei, Guoke

AU - Simionesie, Dorin

AU - Sefcik, Jan

AU - Sutter, Jens U.

AU - Xue, Qingjiang

AU - Yu, Jun

AU - Wang, Jinliang

AU - Birch, David J.S.

AU - Chen, Yu

PY - 2015/12/15

Y1 - 2015/12/15

N2 - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modeling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for realtime monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

AB - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modeling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for realtime monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

UR - https://www.scopus.com/pages/publications/84956695398

U2 - 10.1364/OL.40.005738

DO - 10.1364/OL.40.005738

M3 - 文章

C2 - 26670500

AN - SCOPUS:84956695398

SN - 0146-9592

VL - 40

SP - 5738

EP - 5741

JO - Optics Letters

JF - Optics Letters

IS - 24

ER -

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Abstract

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