Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus

Y. N. Yang; N. Ren; J. M. Xue; J. Yang; B. L. Rong

doi:10.1016/j.nimb.2007.05.016

Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus

Y. N. Yang^*
, N. Ren
, J. M. Xue
, J. Yang
, B. L. Rong

^*Corresponding author for this work

School of Space and Earth Sciences

Beihang University
Peking University

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A 3.2 MeV proton beam was used to irradiate bioflocculant bacteria (Bacillus cereus) to achieve mutation. The ion fluence ranged from 10¹¹ to 10¹⁴/cm². Most of the bacteria were killed when the ion fluence reached 10¹² ions/cm². The survival ratio drops in an exponential way on further increasing the ion fluence. The flocculating activity of 7 samples out of 51 showed a positive change, and a perfect mutant C7-23 with a stable high capacity of bioflocculant production was found. RAPD measurements showed that a new lane appears in this sample. The flocculating activity of the C7-23 bacteria increased by factors of 22%, 54% and 217% under pH values of 4, 7 or 10, respectively.

Original language	English
Pages (from-to)	220-224
Number of pages	5
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	262
Issue number	2
DOIs	https://doi.org/10.1016/j.nimb.2007.05.016
State	Published - Sep 2007

Keywords

Bioflocculant
Heavy ions
Irradiation
RAPD
Survival ratio

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10.1016/j.nimb.2007.05.016

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title = "Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus",

abstract = "A 3.2 MeV proton beam was used to irradiate bioflocculant bacteria (Bacillus cereus) to achieve mutation. The ion fluence ranged from 1011 to 1014/cm2. Most of the bacteria were killed when the ion fluence reached 1012 ions/cm2. The survival ratio drops in an exponential way on further increasing the ion fluence. The flocculating activity of 7 samples out of 51 showed a positive change, and a perfect mutant C7-23 with a stable high capacity of bioflocculant production was found. RAPD measurements showed that a new lane appears in this sample. The flocculating activity of the C7-23 bacteria increased by factors of 22\%, 54\% and 217\% under pH values of 4, 7 or 10, respectively.",

keywords = "Bioflocculant, Heavy ions, Irradiation, RAPD, Survival ratio",

author = "Yang, \{Y. N.\} and N. Ren and Xue, \{J. M.\} and J. Yang and Rong, \{B. L.\}",

year = "2007",

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doi = "10.1016/j.nimb.2007.05.016",

language = "英语",

volume = "262",

pages = "220--224",

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}

TY - JOUR

T1 - Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus

AU - Yang, Y. N.

AU - Ren, N.

AU - Xue, J. M.

AU - Yang, J.

AU - Rong, B. L.

PY - 2007/9

Y1 - 2007/9

N2 - A 3.2 MeV proton beam was used to irradiate bioflocculant bacteria (Bacillus cereus) to achieve mutation. The ion fluence ranged from 1011 to 1014/cm2. Most of the bacteria were killed when the ion fluence reached 1012 ions/cm2. The survival ratio drops in an exponential way on further increasing the ion fluence. The flocculating activity of 7 samples out of 51 showed a positive change, and a perfect mutant C7-23 with a stable high capacity of bioflocculant production was found. RAPD measurements showed that a new lane appears in this sample. The flocculating activity of the C7-23 bacteria increased by factors of 22%, 54% and 217% under pH values of 4, 7 or 10, respectively.

AB - A 3.2 MeV proton beam was used to irradiate bioflocculant bacteria (Bacillus cereus) to achieve mutation. The ion fluence ranged from 1011 to 1014/cm2. Most of the bacteria were killed when the ion fluence reached 1012 ions/cm2. The survival ratio drops in an exponential way on further increasing the ion fluence. The flocculating activity of 7 samples out of 51 showed a positive change, and a perfect mutant C7-23 with a stable high capacity of bioflocculant production was found. RAPD measurements showed that a new lane appears in this sample. The flocculating activity of the C7-23 bacteria increased by factors of 22%, 54% and 217% under pH values of 4, 7 or 10, respectively.

KW - Bioflocculant

KW - Heavy ions

KW - Irradiation

KW - RAPD

KW - Survival ratio

UR - https://www.scopus.com/pages/publications/34547958418

U2 - 10.1016/j.nimb.2007.05.016

DO - 10.1016/j.nimb.2007.05.016

M3 - 文章

AN - SCOPUS:34547958418

SN - 0168-583X

VL - 262

SP - 220

EP - 224

JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

IS - 2

ER -

Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus

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Keywords

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