Abstract
Heterogeneities and oncogenesis essentially result from proteomic disorders orchestrated by changes in DNA and/or cytoplasmic mRNA. These genetic fluctuations, however, cannot be decoded through conventional label-free methods (e.g., patch clamps, electrochemical cellular biosensors, etc.) or morphological characterization. Molecular beacons (MBs) have recently emerged as efficient probes for interrogating biomarkers in live cancer cells. MBs hybridize with their intracellular targets (e.g., mRNAs, DNAs, or proteins), emitting a fluorescent signal that can be quantified and correlated with the expression levels of their targets. In this review we discuss MB probes with different delivery platforms for intracellular probing as well as novel MB designs for detecting a variety of targets in living cancer cells. Finally, we describe current trends in MB-based intracellular biosensors.
| Original language | English |
|---|---|
| Pages (from-to) | 347-359 |
| Number of pages | 13 |
| Journal | Trends in Biotechnology |
| Volume | 35 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1 Apr 2017 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- cancer cells
- heterogeneities
- intracellular delivery
- intracellular probes
- mRNAs
- molecular beacons
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