Microscopic vision based on the adaptive positioning of the camera coordinate frame

Microscopic vision measurement precision has been largely limited by inaccurately calibrated model parameters, because image plane is near parallel to reference plane in the narrow depth of field. This article proposes a method of precise microscopic vision measurement based on the adaptive positioning of the camera coordinate frame. The microscopic vision measurement movably attaches the origin of the camera coordinate frame along the optical axis. By finding the optimal position, the nonlinearity of the objective function in calibration optimization is decreased and the optimization sensitivity to initial values is reduced. Therefore, we obtain a high calibration precision and eventually ensure a high measurement precision. Mathematical simulations illustrate that the calibration precision of the proposed microscopic vision measurement model is higher than that of the conventional vision measurement model. The experiment shows that with magnification of 3.024×, the presented system achieves a precision of 0.12% based on the proposed microscopic vision measurement model, which is two times higher than the one based on the conventional vision measurement model.