Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement

Conghao Wang; Huilan Liu; Jianrui Ma; Qiang Fu; Yijun Li; Yanchuan Chen; Yuqian Gao; Jingquan Tian; Xinlei Luo; Fei Yu; Chunzhu Zhao; Runlong Wu; Aimin Wang; Lishuang Feng

doi:10.3390/photonics10030342

Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement

Conghao Wang
, Huilan Liu
, Jianrui Ma
, Qiang Fu
, Yijun Li
, Yanchuan Chen
, Yuqian Gao
, Jingquan Tian
, Xinlei Luo
, Fei Yu
, Chunzhu Zhao
, Runlong Wu
, Aimin Wang
, Lishuang Feng^*

^*Corresponding author for this work

School of Instrumentation and Optoelectronic Engineering

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Two-photon endomicroscopy is a promising technique with the ability to achieve in situ imaging and diagnosis at subcellular resolution. The large field-of-view capability is essential and useful to locate and image suspicious areas of biological tissue. In this work, we report objective-lens-free, lensed fiber-optic two-photon endomicroscopy for field-of-view enhancement. The field of view of this two-photon endomicroscopic probe is 750 μm with a resolution of 3.03 μm. This 1.6 g miniature probe has an integrated outer diameter of 5.8 mm and a rigid length of 33.5 mm. The imaging performance of the lensed-fiber-optic two-photon endomicroscopy was validated by examining an ex vivo mouse heart, kidney, brain, stomach wall tissues, and in vivo brain tissue.

Original language	English
Article number	342
Journal	Photonics
Volume	10
Issue number	3
DOIs	https://doi.org/10.3390/photonics10030342
State	Published - Mar 2023

Keywords

field-of-view enhancement
lensed fiber
two-photon endomicroscopy

Access to Document

10.3390/photonics10030342

Cite this

@article{ba447b0189d34a7188478df09ba5e009,

title = "Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement",

abstract = "Two-photon endomicroscopy is a promising technique with the ability to achieve in situ imaging and diagnosis at subcellular resolution. The large field-of-view capability is essential and useful to locate and image suspicious areas of biological tissue. In this work, we report objective-lens-free, lensed fiber-optic two-photon endomicroscopy for field-of-view enhancement. The field of view of this two-photon endomicroscopic probe is 750 μm with a resolution of 3.03 μm. This 1.6 g miniature probe has an integrated outer diameter of 5.8 mm and a rigid length of 33.5 mm. The imaging performance of the lensed-fiber-optic two-photon endomicroscopy was validated by examining an ex vivo mouse heart, kidney, brain, stomach wall tissues, and in vivo brain tissue.",

keywords = "field-of-view enhancement, lensed fiber, two-photon endomicroscopy",

author = "Conghao Wang and Huilan Liu and Jianrui Ma and Qiang Fu and Yijun Li and Yanchuan Chen and Yuqian Gao and Jingquan Tian and Xinlei Luo and Fei Yu and Chunzhu Zhao and Runlong Wu and Aimin Wang and Lishuang Feng",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = mar,

doi = "10.3390/photonics10030342",

language = "英语",

volume = "10",

journal = "Photonics",

issn = "2304-6732",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "3",

}

TY - JOUR

T1 - Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement

AU - Wang, Conghao

AU - Liu, Huilan

AU - Ma, Jianrui

AU - Fu, Qiang

AU - Li, Yijun

AU - Chen, Yanchuan

AU - Gao, Yuqian

AU - Tian, Jingquan

AU - Luo, Xinlei

AU - Yu, Fei

AU - Zhao, Chunzhu

AU - Wu, Runlong

AU - Wang, Aimin

AU - Feng, Lishuang

PY - 2023/3

Y1 - 2023/3

N2 - Two-photon endomicroscopy is a promising technique with the ability to achieve in situ imaging and diagnosis at subcellular resolution. The large field-of-view capability is essential and useful to locate and image suspicious areas of biological tissue. In this work, we report objective-lens-free, lensed fiber-optic two-photon endomicroscopy for field-of-view enhancement. The field of view of this two-photon endomicroscopic probe is 750 μm with a resolution of 3.03 μm. This 1.6 g miniature probe has an integrated outer diameter of 5.8 mm and a rigid length of 33.5 mm. The imaging performance of the lensed-fiber-optic two-photon endomicroscopy was validated by examining an ex vivo mouse heart, kidney, brain, stomach wall tissues, and in vivo brain tissue.

AB - Two-photon endomicroscopy is a promising technique with the ability to achieve in situ imaging and diagnosis at subcellular resolution. The large field-of-view capability is essential and useful to locate and image suspicious areas of biological tissue. In this work, we report objective-lens-free, lensed fiber-optic two-photon endomicroscopy for field-of-view enhancement. The field of view of this two-photon endomicroscopic probe is 750 μm with a resolution of 3.03 μm. This 1.6 g miniature probe has an integrated outer diameter of 5.8 mm and a rigid length of 33.5 mm. The imaging performance of the lensed-fiber-optic two-photon endomicroscopy was validated by examining an ex vivo mouse heart, kidney, brain, stomach wall tissues, and in vivo brain tissue.

KW - field-of-view enhancement

KW - lensed fiber

KW - two-photon endomicroscopy

UR - https://www.scopus.com/pages/publications/85151449412

U2 - 10.3390/photonics10030342

DO - 10.3390/photonics10030342

M3 - 文章

AN - SCOPUS:85151449412

SN - 2304-6732

VL - 10

JO - Photonics

JF - Photonics

IS - 3

M1 - 342

ER -

Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this