Isolation and characterization of microsatellite markers from Brown Eared-pheasant (Crossoptilon mantchuricum)

Yuming Fu; Suping Li; Dongming Li; Minglu Wu; Yuefeng Wu

doi:10.1007/s10592-009-9876-2

Isolation and characterization of microsatellite markers from Brown Eared-pheasant (Crossoptilon mantchuricum)

Yuming Fu
, Suping Li
, Dongming Li
, Minglu Wu
, Yuefeng Wu^*

^*Corresponding author for this work

School of Biological Science and Medical Engineering

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The Brown Eared-pheasant (Crossoptilon mantchuricum) is an endangered species endemic to China. Here we developed eight microsatellite loci using a modified biotin-capture method. In the analyses of 28 individuals sampled, the number of alleles per locus ranged from four to nine. The expected (H_E) and observed (H_O) heterozygosities were 0.466-0.825 and 0.619-0.847, respectively. Results that eight microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as genetic diversity and population genetic structure of C. mantchuricum.

Original language	English
Pages (from-to)	1993-1995
Number of pages	3
Journal	Conservation Genetics
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/s10592-009-9876-2
State	Published - Nov 2009

Keywords

Crossoptilon mantchuricum
Genetic marker
Microsatellite
Polymorphism

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10.1007/s10592-009-9876-2

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title = "Isolation and characterization of microsatellite markers from Brown Eared-pheasant (Crossoptilon mantchuricum)",

abstract = "The Brown Eared-pheasant (Crossoptilon mantchuricum) is an endangered species endemic to China. Here we developed eight microsatellite loci using a modified biotin-capture method. In the analyses of 28 individuals sampled, the number of alleles per locus ranged from four to nine. The expected (HE) and observed (HO) heterozygosities were 0.466-0.825 and 0.619-0.847, respectively. Results that eight microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as genetic diversity and population genetic structure of C. mantchuricum.",

keywords = "Crossoptilon mantchuricum, Genetic marker, Microsatellite, Polymorphism",

author = "Yuming Fu and Suping Li and Dongming Li and Minglu Wu and Yuefeng Wu",

year = "2009",

month = nov,

doi = "10.1007/s10592-009-9876-2",

language = "英语",

volume = "10",

pages = "1993--1995",

journal = "Conservation Genetics",

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TY - JOUR

T1 - Isolation and characterization of microsatellite markers from Brown Eared-pheasant (Crossoptilon mantchuricum)

AU - Fu, Yuming

AU - Li, Suping

AU - Li, Dongming

AU - Wu, Minglu

AU - Wu, Yuefeng

PY - 2009/11

Y1 - 2009/11

N2 - The Brown Eared-pheasant (Crossoptilon mantchuricum) is an endangered species endemic to China. Here we developed eight microsatellite loci using a modified biotin-capture method. In the analyses of 28 individuals sampled, the number of alleles per locus ranged from four to nine. The expected (HE) and observed (HO) heterozygosities were 0.466-0.825 and 0.619-0.847, respectively. Results that eight microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as genetic diversity and population genetic structure of C. mantchuricum.

AB - The Brown Eared-pheasant (Crossoptilon mantchuricum) is an endangered species endemic to China. Here we developed eight microsatellite loci using a modified biotin-capture method. In the analyses of 28 individuals sampled, the number of alleles per locus ranged from four to nine. The expected (HE) and observed (HO) heterozygosities were 0.466-0.825 and 0.619-0.847, respectively. Results that eight microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as genetic diversity and population genetic structure of C. mantchuricum.

KW - Crossoptilon mantchuricum

KW - Genetic marker

KW - Microsatellite

KW - Polymorphism

UR - https://www.scopus.com/pages/publications/71349086592

U2 - 10.1007/s10592-009-9876-2

DO - 10.1007/s10592-009-9876-2

M3 - 文章

AN - SCOPUS:71349086592

SN - 1566-0621

VL - 10

SP - 1993

EP - 1995

JO - Conservation Genetics

JF - Conservation Genetics

IS - 6

ER -

Isolation and characterization of microsatellite markers from Brown Eared-pheasant (Crossoptilon mantchuricum)

Abstract

Keywords

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