Influence of TiO2 nanoparticles on glutathione in rat synovial cell line RSC-364

Jiangxue Wang; Ying Hou; Linmeng Dong; Xufeng Niu; Yubo Fan

doi:10.1007/978-3-642-29305-4_21

Influence of TiO₂ nanoparticles on glutathione in rat synovial cell line RSC-364

Jiangxue Wang
, Ying Hou
, Linmeng Dong
, Xufeng Niu
, Yubo Fan^*

^*Corresponding author for this work

Beihang University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Nanobiomaterials have good prospect in application of biomedical products and orthopedic implants. However, increasing evidence suggest that the special physicochemical properties of nanobiomaterials pose potential risks to human health. For determining its cytotoxicity on rat synovial cell line (RSC-364), in this study, the effect of TiO₂ nanoparticles(nano-TiO₂) on glutathione in cells was evaluated. By incubating RSC-364 cells with nano-TiO₂ in different concentration, the over-production of reactive oxygen species was determined by flow cytometry. The intracellular glutathione(GSH), glutathione- related antioxidants contents were assayed using biochemical methods to estimate the oxidative damage in cells. Total glutathione (T-GSH) and reduced GSH contents decreased in RSC-364 cells after exposure to nano-TiO₂. This was in dose-dependent relationship. The oxidized glutathione level decreased too, indicating the serious damage in oxidant/ antioxidant system. Results implied that nano-TiO₂ exposure caused the oxidative stress in RSC-364 cells by overproduced free radicals. The cellular redox status was interrupted.

Original language	English
Title of host publication	World Congress on Medical Physics and Biomedical Engineering
Pages	75-78
Number of pages	4
DOIs	https://doi.org/10.1007/978-3-642-29305-4_21
State	Published - 2013
Event	World Congress on Medical Physics and Biomedical Engineering - Beijing, China Duration: 26 May 2012 → 31 May 2012

Publication series

Name	IFMBE Proceedings
Volume	39 IFMBE
ISSN (Print)	1680-0737

Conference

Conference	World Congress on Medical Physics and Biomedical Engineering
Country/Territory	China
City	Beijing
Period	26/05/12 → 31/05/12

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

GSH
TiO nanoparticles
oxidative stress
rat synovial cell
reactive oxygen species

Access to Document

10.1007/978-3-642-29305-4_21

Cite this

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title = "Influence of TiO2 nanoparticles on glutathione in rat synovial cell line RSC-364",

abstract = "Nanobiomaterials have good prospect in application of biomedical products and orthopedic implants. However, increasing evidence suggest that the special physicochemical properties of nanobiomaterials pose potential risks to human health. For determining its cytotoxicity on rat synovial cell line (RSC-364), in this study, the effect of TiO2 nanoparticles(nano-TiO2) on glutathione in cells was evaluated. By incubating RSC-364 cells with nano-TiO2 in different concentration, the over-production of reactive oxygen species was determined by flow cytometry. The intracellular glutathione(GSH), glutathione- related antioxidants contents were assayed using biochemical methods to estimate the oxidative damage in cells. Total glutathione (T-GSH) and reduced GSH contents decreased in RSC-364 cells after exposure to nano-TiO2. This was in dose-dependent relationship. The oxidized glutathione level decreased too, indicating the serious damage in oxidant/ antioxidant system. Results implied that nano-TiO2 exposure caused the oxidative stress in RSC-364 cells by overproduced free radicals. The cellular redox status was interrupted.",

keywords = "GSH, TiO nanoparticles, oxidative stress, rat synovial cell, reactive oxygen species",

author = "Jiangxue Wang and Ying Hou and Linmeng Dong and Xufeng Niu and Yubo Fan",

year = "2013",

doi = "10.1007/978-3-642-29305-4\_21",

language = "英语",

isbn = "9783642293047",

series = "IFMBE Proceedings",

pages = "75--78",

booktitle = "World Congress on Medical Physics and Biomedical Engineering",

note = "World Congress on Medical Physics and Biomedical Engineering ; Conference date: 26-05-2012 Through 31-05-2012",

}

Wang, J, Hou, Y, Dong, L, Niu, X & Fan, Y 2013, Influence of TiO₂ nanoparticles on glutathione in rat synovial cell line RSC-364. in World Congress on Medical Physics and Biomedical Engineering. IFMBE Proceedings, vol. 39 IFMBE, pp. 75-78, World Congress on Medical Physics and Biomedical Engineering, Beijing, China, 26/05/12. https://doi.org/10.1007/978-3-642-29305-4_21

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AU - Wang, Jiangxue

AU - Hou, Ying

AU - Dong, Linmeng

AU - Niu, Xufeng

AU - Fan, Yubo

PY - 2013

Y1 - 2013

N2 - Nanobiomaterials have good prospect in application of biomedical products and orthopedic implants. However, increasing evidence suggest that the special physicochemical properties of nanobiomaterials pose potential risks to human health. For determining its cytotoxicity on rat synovial cell line (RSC-364), in this study, the effect of TiO2 nanoparticles(nano-TiO2) on glutathione in cells was evaluated. By incubating RSC-364 cells with nano-TiO2 in different concentration, the over-production of reactive oxygen species was determined by flow cytometry. The intracellular glutathione(GSH), glutathione- related antioxidants contents were assayed using biochemical methods to estimate the oxidative damage in cells. Total glutathione (T-GSH) and reduced GSH contents decreased in RSC-364 cells after exposure to nano-TiO2. This was in dose-dependent relationship. The oxidized glutathione level decreased too, indicating the serious damage in oxidant/ antioxidant system. Results implied that nano-TiO2 exposure caused the oxidative stress in RSC-364 cells by overproduced free radicals. The cellular redox status was interrupted.

AB - Nanobiomaterials have good prospect in application of biomedical products and orthopedic implants. However, increasing evidence suggest that the special physicochemical properties of nanobiomaterials pose potential risks to human health. For determining its cytotoxicity on rat synovial cell line (RSC-364), in this study, the effect of TiO2 nanoparticles(nano-TiO2) on glutathione in cells was evaluated. By incubating RSC-364 cells with nano-TiO2 in different concentration, the over-production of reactive oxygen species was determined by flow cytometry. The intracellular glutathione(GSH), glutathione- related antioxidants contents were assayed using biochemical methods to estimate the oxidative damage in cells. Total glutathione (T-GSH) and reduced GSH contents decreased in RSC-364 cells after exposure to nano-TiO2. This was in dose-dependent relationship. The oxidized glutathione level decreased too, indicating the serious damage in oxidant/ antioxidant system. Results implied that nano-TiO2 exposure caused the oxidative stress in RSC-364 cells by overproduced free radicals. The cellular redox status was interrupted.

KW - GSH

KW - TiO nanoparticles

KW - oxidative stress

KW - rat synovial cell

KW - reactive oxygen species

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