Evidencing the uptake of electrons from X80 steel by Bacillus licheniformis with redox probe, 5-cyano-2,3-ditolyl tetrazolium chloride

Jun Li; Zhiyong Liu; Yuntian Lou; Cuiwei Du; Xiaogang Li

doi:10.1016/j.corsci.2020.108569

Evidencing the uptake of electrons from X80 steel by Bacillus licheniformis with redox probe, 5-cyano-2,3-ditolyl tetrazolium chloride

Jun Li
, Zhiyong Liu^*
, Yuntian Lou
, Cuiwei Du
, Xiaogang Li

^*Corresponding author for this work

University of Science and Technology Beijing
Key Laboratory of Precision Opto-Mechatronics Technology (Ministry of Education)

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon starvation with CTC/DAPI counterstaining and developed a novel method for detecting EET from metal substrate to the cells. A wild-type nitrate-reducing bacterium (NRB) Bacillus licheniformis was evidenced to be able to take up electrons from the X80 steel substrate for respiration. This study provides reliable support to the bioenergetic theory for microbiologically influenced corrosion (MIC) of carbon steel by NRB.

Original language	English
Article number	108569
Journal	Corrosion Science
Volume	168
DOIs	https://doi.org/10.1016/j.corsci.2020.108569
State	Published - 15 May 2020
Externally published	Yes

Keywords

Bacillus licheniformis
Extracellular electron transfer
Microbiologically influenced corrosion
Redox probe
X80 pipeline steel

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10.1016/j.corsci.2020.108569

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title = "Evidencing the uptake of electrons from X80 steel by Bacillus licheniformis with redox probe, 5-cyano-2,3-ditolyl tetrazolium chloride",

abstract = "Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon starvation with CTC/DAPI counterstaining and developed a novel method for detecting EET from metal substrate to the cells. A wild-type nitrate-reducing bacterium (NRB) Bacillus licheniformis was evidenced to be able to take up electrons from the X80 steel substrate for respiration. This study provides reliable support to the bioenergetic theory for microbiologically influenced corrosion (MIC) of carbon steel by NRB.",

keywords = "Bacillus licheniformis, Extracellular electron transfer, Microbiologically influenced corrosion, Redox probe, X80 pipeline steel",

author = "Jun Li and Zhiyong Liu and Yuntian Lou and Cuiwei Du and Xiaogang Li",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = may,

day = "15",

doi = "10.1016/j.corsci.2020.108569",

language = "英语",

volume = "168",

journal = "Corrosion Science",

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TY - JOUR

T1 - Evidencing the uptake of electrons from X80 steel by Bacillus licheniformis with redox probe, 5-cyano-2,3-ditolyl tetrazolium chloride

AU - Li, Jun

AU - Liu, Zhiyong

AU - Lou, Yuntian

AU - Du, Cuiwei

AU - Li, Xiaogang

PY - 2020/5/15

Y1 - 2020/5/15

N2 - Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon starvation with CTC/DAPI counterstaining and developed a novel method for detecting EET from metal substrate to the cells. A wild-type nitrate-reducing bacterium (NRB) Bacillus licheniformis was evidenced to be able to take up electrons from the X80 steel substrate for respiration. This study provides reliable support to the bioenergetic theory for microbiologically influenced corrosion (MIC) of carbon steel by NRB.

AB - Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon starvation with CTC/DAPI counterstaining and developed a novel method for detecting EET from metal substrate to the cells. A wild-type nitrate-reducing bacterium (NRB) Bacillus licheniformis was evidenced to be able to take up electrons from the X80 steel substrate for respiration. This study provides reliable support to the bioenergetic theory for microbiologically influenced corrosion (MIC) of carbon steel by NRB.

KW - Bacillus licheniformis

KW - Extracellular electron transfer

KW - Microbiologically influenced corrosion

KW - Redox probe

KW - X80 pipeline steel

UR - https://www.scopus.com/pages/publications/85081548002

U2 - 10.1016/j.corsci.2020.108569

DO - 10.1016/j.corsci.2020.108569

M3 - 文章

AN - SCOPUS:85081548002

SN - 0010-938X

VL - 168

JO - Corrosion Science

JF - Corrosion Science

M1 - 108569

ER -

Evidencing the uptake of electrons from X80 steel by Bacillus licheniformis with redox probe, 5-cyano-2,3-ditolyl tetrazolium chloride

Abstract

Keywords

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