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Electrochemical aptasensor for L-histidine detection via self-cleavage of DNAzymes and amplification of end-truncated elongated tetrahexaheral gold nanocrystals

  • Zhengbo Chen
  • , Xiaojiao Mu
  • , Jing Li
  • , Lidong Li
  • , Lin Guo*
  • *Corresponding author for this work
  • Beihang University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A sensitive aptamer-based signal-on sensor for real-time detection of L-histidine via selfcleavage of DNAzymes and amplification of elongated tetrahexaheral gold nanocrystals (endtruncated-ETHH-Au-NCs) is presented. The introduction of target L-histidine induced selfcleavage of DNAzymes on the end-truncated-ETHH-Au-NCs modified glassy carbon electrode (GCE) into the solution. The resulting change in electron transfer efficiency is readily measured by square wave voltammetry (SWV) at target L-histidine concentrations as low as 0.1 pM, the increase in the SWV peak current is linear with the concentration of L-histidine in the range from 0.1 pM to 0.1 μM. The fabricated aptasensor shows a high specificity for L-histidine which is not affected by the coexistence of other amino acids, and an excellent enantio-selectivity toward L-histidine was achieved.

Original languageEnglish
Title of host publicationAdvanced Research on Material Engineering and Its Application
PublisherTrans Tech Publications Ltd
Pages213-216
Number of pages4
ISBN (Print)9783037853740
DOIs
StatePublished - 2012
Event2012 2nd International Conference on Information Science, Automation and Material System, ISAM 2012 - Changsha, China
Duration: 21 Apr 201222 Apr 2012

Publication series

NameApplied Mechanics and Materials
Volume485
ISSN (Print)1660-9336
ISSN (Electronic)1662-7482

Conference

Conference2012 2nd International Conference on Information Science, Automation and Material System, ISAM 2012
Country/TerritoryChina
CityChangsha
Period21/04/1222/04/12

Keywords

  • Aptasensor
  • Electrochemistry
  • End-truncated-ETHH-Au-NCs
  • L-histidine
  • Self-cleavage

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