Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

Qi Zhang; Yan Xiang; Dawei Liang; Yinggui Peng; Hong Guo

doi:10.1016/j.bmcl.2011.11.028

Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

Qi Zhang
, Yan Xiang^*
, Dawei Liang
, Yinggui Peng
, Hong Guo

^*Corresponding author for this work

Beihang University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (k_obs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 10⁷-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.

Original language	English
Pages (from-to)	1814-1817
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	22
Issue number	4
DOIs	https://doi.org/10.1016/j.bmcl.2011.11.028
State	Published - 15 Feb 2012

Keywords

Artificial nuclease
Chitosan
DNA cleavage
Double-strand
Zinc complex

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10.1016/j.bmcl.2011.11.028

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title = "Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex",

abstract = "N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (kobs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 107-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.",

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TY - JOUR

T1 - Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

AU - Zhang, Qi

AU - Xiang, Yan

AU - Liang, Dawei

AU - Peng, Yinggui

AU - Guo, Hong

PY - 2012/2/15

Y1 - 2012/2/15

N2 - N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (kobs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 107-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.

AB - N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (kobs) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 107-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA.

KW - Artificial nuclease

KW - Chitosan

KW - DNA cleavage

KW - Double-strand

KW - Zinc complex

UR - https://www.scopus.com/pages/publications/84862793026

U2 - 10.1016/j.bmcl.2011.11.028

DO - 10.1016/j.bmcl.2011.11.028

M3 - 文章

AN - SCOPUS:84862793026

SN - 0960-894X

VL - 22

SP - 1814

EP - 1817

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 4

ER -

Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex

Abstract

Keywords

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