Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro

Ping Li; Yu Li; Lizhen Liu; Lu Jia; Yanhui Song; Xiaoling Jia; Yubo Fan

doi:10.1007/978-3-642-29305-4_51

Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro

Ping Li
, Yu Li
, Lizhen Liu
, Lu Jia
, Yanhui Song
, Xiaoling Jia
, Yubo Fan^*

^*Corresponding author for this work

Beihang University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissue and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase II combined digestion. After 72h's culture, different electrical stimulations (0.5V/cm, 1V/cm, 2V/cm; 1Hz; 2ms; 30min) and mechanical stretching force (tensile 10%, 6h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5V/cm was used to apply to cardiomyocytes simultaneously. After stimulation, Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5V/cm resulted in obvious enhancement of Cx-43 mRNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10% of tensile stimulation for 6h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation, which is more beneficial to the development and connection of cardiomyocytes.

Original language	English
Title of host publication	World Congress on Medical Physics and Biomedical Engineering
Pages	188-191
Number of pages	4
DOIs	https://doi.org/10.1007/978-3-642-29305-4_51
State	Published - 2013
Event	World Congress on Medical Physics and Biomedical Engineering - Beijing, China Duration: 26 May 2012 → 31 May 2012

Publication series

Name	IFMBE Proceedings
Volume	39 IFMBE
ISSN (Print)	1680-0737

Conference

Conference	World Congress on Medical Physics and Biomedical Engineering
Country/Territory	China
City	Beijing
Period	26/05/12 → 31/05/12

Keywords

cardiomyocytes
combinative effects
Connexin-43 mRNA
electrical stimulation
tensile stimulation

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10.1007/978-3-642-29305-4_51

Cite this

@inproceedings{dd646335a0104d0b840c32cc8aa7caa8,

title = "Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro",

abstract = "The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissue and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase II combined digestion. After 72h's culture, different electrical stimulations (0.5V/cm, 1V/cm, 2V/cm; 1Hz; 2ms; 30min) and mechanical stretching force (tensile 10\%, 6h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5V/cm was used to apply to cardiomyocytes simultaneously. After stimulation, Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5V/cm resulted in obvious enhancement of Cx-43 mRNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10\% of tensile stimulation for 6h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation, which is more beneficial to the development and connection of cardiomyocytes.",

keywords = "cardiomyocytes, combinative effects, Connexin-43 mRNA, electrical stimulation, tensile stimulation",

author = "Ping Li and Yu Li and Lizhen Liu and Lu Jia and Yanhui Song and Xiaoling Jia and Yubo Fan",

year = "2013",

doi = "10.1007/978-3-642-29305-4\_51",

language = "英语",

isbn = "9783642293047",

series = "IFMBE Proceedings",

pages = "188--191",

booktitle = "World Congress on Medical Physics and Biomedical Engineering",

note = "World Congress on Medical Physics and Biomedical Engineering ; Conference date: 26-05-2012 Through 31-05-2012",

}

Li, P, Li, Y, Liu, L, Jia, L, Song, Y, Jia, X & Fan, Y 2013, Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro. in World Congress on Medical Physics and Biomedical Engineering. IFMBE Proceedings, vol. 39 IFMBE, pp. 188-191, World Congress on Medical Physics and Biomedical Engineering, Beijing, China, 26/05/12. https://doi.org/10.1007/978-3-642-29305-4_51

Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro. / Li, Ping; Li, Yu; Liu, Lizhen et al.
World Congress on Medical Physics and Biomedical Engineering. 2013. p. 188-191 (IFMBE Proceedings; Vol. 39 IFMBE).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro

AU - Li, Ping

AU - Li, Yu

AU - Liu, Lizhen

AU - Jia, Lu

AU - Song, Yanhui

AU - Jia, Xiaoling

AU - Fan, Yubo

PY - 2013

Y1 - 2013

N2 - The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissue and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase II combined digestion. After 72h's culture, different electrical stimulations (0.5V/cm, 1V/cm, 2V/cm; 1Hz; 2ms; 30min) and mechanical stretching force (tensile 10%, 6h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5V/cm was used to apply to cardiomyocytes simultaneously. After stimulation, Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5V/cm resulted in obvious enhancement of Cx-43 mRNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10% of tensile stimulation for 6h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation, which is more beneficial to the development and connection of cardiomyocytes.

AB - The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissue and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase II combined digestion. After 72h's culture, different electrical stimulations (0.5V/cm, 1V/cm, 2V/cm; 1Hz; 2ms; 30min) and mechanical stretching force (tensile 10%, 6h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5V/cm was used to apply to cardiomyocytes simultaneously. After stimulation, Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5V/cm resulted in obvious enhancement of Cx-43 mRNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10% of tensile stimulation for 6h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation, which is more beneficial to the development and connection of cardiomyocytes.

KW - cardiomyocytes

KW - combinative effects

KW - Connexin-43 mRNA

KW - electrical stimulation

KW - tensile stimulation

UR - https://www.scopus.com/pages/publications/84876035844

U2 - 10.1007/978-3-642-29305-4_51

DO - 10.1007/978-3-642-29305-4_51

M3 - 会议稿件

AN - SCOPUS:84876035844

SN - 9783642293047

T3 - IFMBE Proceedings

SP - 188

EP - 191

BT - World Congress on Medical Physics and Biomedical Engineering

T2 - World Congress on Medical Physics and Biomedical Engineering

Y2 - 26 May 2012 through 31 May 2012

ER -

Effects of tensile stimulation and electrical stimulation on the connexion-43 mRNA levels of cardiomyocytes in vitro

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