Development of localized surface plasmon resonance based biosensor using Au deposited nano-porous aluminum anodic oxide chip

In this study, we fabricated a highly sensitive and real-time monitoring biosensor using a nanoporous aluminum anodic oxide (AAO) chip. We improved the surface uniformity by two-step anodizing and fabricated an AAO chip having a pore size of 50 nm. The device uses interference and localized surface plasmon resonance (LSPR) phenomena, and it has high sensitivity and selectivity relative to a sensing device that use a single sensing mechanism. We deposited gold on an Al₂O₃ layer to induce the LSPR phenomenon, and fabricated a sensing membrane using the SAM method to immobilize troponin T antibody on gold. A real time biosensor system is constructed using optical spectroscopy and an optical fiber reflectance probe. We measured troponin T Antigen quantitatively using fabricated sensor, and confirmed its applicability as a diagnostic tool for myocardial infarction. The troponin T is known as an unusual myocardial injury marker, and as a protein that forms the complexes with troponin I, and troponin C, which control muscle contraction.