Detection of HCG-antigen based on enhanced photoluminescence of hierarchical ZnO arrays

Xiang Yan; Zhongbing Huang; Mian He; Xiaoming Liao; Chaoqun Zhang; Guangfu Yin; Jianwen Gu

doi:10.1016/j.colsurfb.2011.08.027

Detection of HCG-antigen based on enhanced photoluminescence of hierarchical ZnO arrays

Xiang Yan
, Zhongbing Huang^*
, Mian He
, Xiaoming Liao
, Chaoqun Zhang
, Guangfu Yin
, Jianwen Gu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Specific antibody of Human chorionic gonadotrophin (HCG) was conjugated onto hierarchical ZnO arrays through the carbodiimide technique, and the photoluminescence (PL) intensity of ZnO arrays were enhanced linearly with the linked antibody concentration in the range of 40-160. ng/mL, which resulted from the nano sheet structure and rough surface in hierarchical ZnO columns. After the specific combination between antigen and antibody on hierarchical ZnO arrays, the enhanced PL intensity of ZnO arrays was also basically linear with the concentration of HCG antigen. Thus, detection of HCG antigen in the range of 2-20. ng/mL was achieved based on PL intensity enhancement, suggesting that the prepared ZnO arrays are envisioned to be applied in the detection of early tumor markers in future.

Original language	English
Pages (from-to)	86-92
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2011.08.027
State	Published - 1 Jan 2012
Externally published	Yes

Keywords

Early detection
HCG antibody-antigen
Hierarchical ZnO column arrays
Photoluminescence

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10.1016/j.colsurfb.2011.08.027

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title = "Detection of HCG-antigen based on enhanced photoluminescence of hierarchical ZnO arrays",

abstract = "Specific antibody of Human chorionic gonadotrophin (HCG) was conjugated onto hierarchical ZnO arrays through the carbodiimide technique, and the photoluminescence (PL) intensity of ZnO arrays were enhanced linearly with the linked antibody concentration in the range of 40-160. ng/mL, which resulted from the nano sheet structure and rough surface in hierarchical ZnO columns. After the specific combination between antigen and antibody on hierarchical ZnO arrays, the enhanced PL intensity of ZnO arrays was also basically linear with the concentration of HCG antigen. Thus, detection of HCG antigen in the range of 2-20. ng/mL was achieved based on PL intensity enhancement, suggesting that the prepared ZnO arrays are envisioned to be applied in the detection of early tumor markers in future.",

keywords = "Early detection, HCG antibody-antigen, Hierarchical ZnO column arrays, Photoluminescence",

author = "Xiang Yan and Zhongbing Huang and Mian He and Xiaoming Liao and Chaoqun Zhang and Guangfu Yin and Jianwen Gu",

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doi = "10.1016/j.colsurfb.2011.08.027",

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TY - JOUR

T1 - Detection of HCG-antigen based on enhanced photoluminescence of hierarchical ZnO arrays

AU - Yan, Xiang

AU - Huang, Zhongbing

AU - He, Mian

AU - Liao, Xiaoming

AU - Zhang, Chaoqun

AU - Yin, Guangfu

AU - Gu, Jianwen

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Specific antibody of Human chorionic gonadotrophin (HCG) was conjugated onto hierarchical ZnO arrays through the carbodiimide technique, and the photoluminescence (PL) intensity of ZnO arrays were enhanced linearly with the linked antibody concentration in the range of 40-160. ng/mL, which resulted from the nano sheet structure and rough surface in hierarchical ZnO columns. After the specific combination between antigen and antibody on hierarchical ZnO arrays, the enhanced PL intensity of ZnO arrays was also basically linear with the concentration of HCG antigen. Thus, detection of HCG antigen in the range of 2-20. ng/mL was achieved based on PL intensity enhancement, suggesting that the prepared ZnO arrays are envisioned to be applied in the detection of early tumor markers in future.

AB - Specific antibody of Human chorionic gonadotrophin (HCG) was conjugated onto hierarchical ZnO arrays through the carbodiimide technique, and the photoluminescence (PL) intensity of ZnO arrays were enhanced linearly with the linked antibody concentration in the range of 40-160. ng/mL, which resulted from the nano sheet structure and rough surface in hierarchical ZnO columns. After the specific combination between antigen and antibody on hierarchical ZnO arrays, the enhanced PL intensity of ZnO arrays was also basically linear with the concentration of HCG antigen. Thus, detection of HCG antigen in the range of 2-20. ng/mL was achieved based on PL intensity enhancement, suggesting that the prepared ZnO arrays are envisioned to be applied in the detection of early tumor markers in future.

KW - Early detection

KW - HCG antibody-antigen

KW - Hierarchical ZnO column arrays

KW - Photoluminescence

UR - https://www.scopus.com/pages/publications/80055003085

U2 - 10.1016/j.colsurfb.2011.08.027

DO - 10.1016/j.colsurfb.2011.08.027

M3 - 文章

C2 - 21944543

AN - SCOPUS:80055003085

SN - 0927-7765

VL - 89

SP - 86

EP - 92

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1

ER -

Detection of HCG-antigen based on enhanced photoluminescence of hierarchical ZnO arrays

Abstract

Keywords

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