Deep Learning Assisted Automated Separation Platform of Single Cells and Microparticles Using Optoelectronic Tweezers

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A deep learning-assisted automated separation platform of single cells and microparticles using optoelectronic tweezers was proposed in this paper, which allows accurate manipulation and long-term dynamic observation of single cells without complex microfluidic structures. A single-cell detector based on YOLOv5 was developed to realize single-cell automation and high throughput recognition in optoelectronic tweezers chips. Then these recognized cells or particles were captured and operated by the light patterns generated by the optoelectronic tweezers system. We built a single-cell separation platform and realized the automatic queuing of disordered microspheres or assigning them to each nearest microchannel in a short time. This work can potentially facilitate the study of cell heterogeneity and biologics drug discovery.

Original languageEnglish
Title of host publicationProceedings of the 4th WRC Symposium on Advanced Robotics and Automation 2022, WRC SARA 2022
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages275-279
Number of pages5
ISBN (Electronic)9781665463690
DOIs
StatePublished - 2022
Event4th WRC Symposium on Advanced Robotics and Automation, WRC SARA 2022 - Beijing, China
Duration: 20 Sep 2022 → …

Publication series

NameProceedings of the 4th WRC Symposium on Advanced Robotics and Automation 2022, WRC SARA 2022

Conference

Conference4th WRC Symposium on Advanced Robotics and Automation, WRC SARA 2022
Country/TerritoryChina
CityBeijing
Period20/09/22 → …

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