Abstract
Red fluorescent protein (RFP) DsRed and its variants are widely applied in live-cell imaging experiments. However, a major factor that restricts the application of DsRed is its cytotoxicity. Here, we report that DsRed and its variant DsRed-Express2 inhibit the expression of B-cell lymphoma-extra large (Bcl-xL) in HeLa cells by translational regulation. Over-expression of Bcl-xL can reduce the cytotoxicity of DsRed. Meanwhile, Turbo RFP, a mutant RFP from Entacmaea quadricolor, does not affect Bcl-xL expression, suggesting that cytotoxic mechanisms of RFP from different species may be varied. Our results reveal a possible mechanism for the cytotoxicity of DsRed, providing a potential strategy to improve the application of DsRed and its variants.
| Original language | English |
|---|---|
| Pages (from-to) | 821-827 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 585 |
| Issue number | 5 |
| DOIs | |
| State | Published - 9 Mar 2011 |
Keywords
- Bcl-xL
- Cytotoxicity
- DsRed
- DsRed-Express2
- Red fluorescent protein
- Turbo RFP
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