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Continuous enucleation of bovine oocyte by microrobot with local flow distribution control

  • Lin Feng*
  • , Akihiko Ichikawa
  • , Fumihito Arai
  • , Masaya Hagiwara
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present a continuous enucleation process of bovine oocytes on a microfluidic chip to achieve the continuous cutting of the oocytes and increasing the potential viability of the enucleated oocyte. By combining microfluidic chip and micororobotics, the flow in a channel can be actively controlled and we achieved successive operations of 1) loading oocyte, 2) control cutting volume and 3) removing nucleus. The magnetically actuated microrobot can control local fluid flow by changing its position like gate valve and control fluid force distributions in a microchip which govern oocyte movement in a chip. The optimally designed microchannel for enucleation enables continuous operation and cutting bovine oocyte with smooth manner by hydrodynamic force. The new system is propose here to show advantages by the means of the continuous operation on oocytes in a short term, cutting precision and great potentiality on continuous enucleation process for clone technology.

Original languageEnglish
Title of host publication2012 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2012 - Conference Proceedings
Pages59-64
Number of pages6
DOIs
StatePublished - 2012
Externally publishedYes
Event2012 2nd International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2012 - Xi'an, China
Duration: 29 Aug 20121 Sep 2012

Publication series

Name2012 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2012 - Conference Proceedings

Conference

Conference2012 2nd International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2012
Country/TerritoryChina
CityXi'an
Period29/08/121/09/12

Keywords

  • Magnetically driven microtool
  • Microfluidic chip
  • Oocyte enucleation
  • microrobot

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