Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke

Ling Yan Leung; Cangel Pui Yee Chan; Yuk Ki Leung; Hui Lin Jiang; Jill Morales Abrigo; De Feng Wang; Joseph Shun Hang Chung; Timothy Hudson Rainer; Colin Alexander Graham

doi:10.1016/j.cca.2014.03.007

Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke

Ling Yan Leung
, Cangel Pui Yee Chan
, Yuk Ki Leung
, Hui Lin Jiang
, Jill Morales Abrigo
, De Feng Wang
, Joseph Shun Hang Chung
, Timothy Hudson Rainer^*
, Colin Alexander Graham

^*Corresponding author for this work

Chinese University of Hong Kong
Guangzhou Medical College
Prince of Wales Hospital Hong Kong

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Background: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke. Methods: miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke. Results: Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×10⁵ vs 2.0×10⁵ copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×10⁹ vs 1.3×10⁹ copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively. Conclusion: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Clinica Chimica Acta
Volume	433
DOIs	https://doi.org/10.1016/j.cca.2014.03.007
State	Published - 10 Jun 2014
Externally published	Yes

Keywords

Diagnosis
Hemorrhagic stroke
Ischemic stroke
MicroRNA
Plasma

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10.1016/j.cca.2014.03.007

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title = "Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke",

abstract = "Background: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke. Methods: miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke. Results: Of 93 patients, 74 (79.6\%) were diagnosed as ischemic stroke (IS) and 19 (20.4\%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×105 vs 2.0×105 copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×109 vs 1.3×109 copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively. Conclusion: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.",

keywords = "Diagnosis, Hemorrhagic stroke, Ischemic stroke, MicroRNA, Plasma",

author = "Leung, \{Ling Yan\} and Chan, \{Cangel Pui Yee\} and Leung, \{Yuk Ki\} and Jiang, \{Hui Lin\} and Abrigo, \{Jill Morales\} and Wang, \{De Feng\} and Chung, \{Joseph Shun Hang\} and Rainer, \{Timothy Hudson\} and Graham, \{Colin Alexander\}",

year = "2014",

month = jun,

day = "10",

doi = "10.1016/j.cca.2014.03.007",

language = "英语",

volume = "433",

pages = "139--144",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke

AU - Leung, Ling Yan

AU - Chan, Cangel Pui Yee

AU - Leung, Yuk Ki

AU - Jiang, Hui Lin

AU - Abrigo, Jill Morales

AU - Wang, De Feng

AU - Chung, Joseph Shun Hang

AU - Rainer, Timothy Hudson

AU - Graham, Colin Alexander

PY - 2014/6/10

Y1 - 2014/6/10

N2 - Background: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke. Methods: miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke. Results: Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×105 vs 2.0×105 copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×109 vs 1.3×109 copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively. Conclusion: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.

AB - Background: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke. Methods: miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke. Results: Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×105 vs 2.0×105 copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×109 vs 1.3×109 copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively. Conclusion: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.

KW - Diagnosis

KW - Hemorrhagic stroke

KW - Ischemic stroke

KW - MicroRNA

KW - Plasma

UR - https://www.scopus.com/pages/publications/84897412976

U2 - 10.1016/j.cca.2014.03.007

DO - 10.1016/j.cca.2014.03.007

M3 - 文章

C2 - 24650689

AN - SCOPUS:84897412976

SN - 0009-8981

VL - 433

SP - 139

EP - 144

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke

Abstract

Keywords

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