Biotemplated Diatom Microrobots for Dual-Drug Delivery in Targeted Neuroblastoma Therapy

Neuroblastoma, a highly malignant pediatric tumor, demands innovative targeted drug delivery strategies to overcome the limitations of conventional therapies. In this study, we developed magnetic diatom microrobots (DMs) using diatom frustules as biotemplates, leveraging their natural hierarchical porosity and biocompatibility. Functionalized with amino groups and folic acid, these microrobots demonstrated enhanced drug conjugation and tumor-targeting capabilities. Designed to carry either cisplatin (CDDP) or paclitaxel (PTX), the DMs facilitated complementary therapeutic effects through pH-responsive release, enabling synergistic neuroblastoma therapy. Magnetic actuation tests confirmed multimodal propulsion and precise motion control. In vitro experiments demonstrated enhanced therapeutic efficacy and reduced off-target effects compared to those of conventional approaches. These diatom-templated magnetic microrobots, with their facile fabrication and superior performance, represent a promising platform for targeted drug delivery and advanced cancer therapy.