Binding characteristics of molecularly imprinted polymer for pazufloxacin

A molecularly imprinted polymer (MIP) with high selectivity was prepared using methacrylic acid (MAA) as monomer, ethylene glycol dimethacrylate (EDMA) as cross-linker and L-pazufloxacin as template. The binding capacity of MIP to the template is higher than that of blank polymer. Scatchard analysis show that the MIP recognized template with two classes of binding sites. The multiple-sites binding model was used to calculate the dissociation constant K_d and apparent maximum number Q_max of binding sites. K_d,1 and Q_max,2of the binding sites with high affinity are 23.08 μmol/L and 3.65 μmol/g, while K_d,2 and Q_max,2 of the binding sites with low affinity are 0.43 mmol/L and 27.28 μmol/g respectively. The selective binding experiment for substrates shows that the affinity and selectivity for pazufloxacin are higher than that for L-norfloxacin and ofloxacin.