Application of the RLGS image analysis tool (RAT) to the construction of a genetic linkage map of recombinant inbred strain SMXA

Yuichi Sugahara; Shingo Akiyoshi; Yasushi Okazaki; Isao Tanihata; Yoshihide Hayashizaki

doi:10.1007/s003359901055

Application of the RLGS image analysis tool (RAT) to the construction of a genetic linkage map of recombinant inbred strain SMXA

Yuichi Sugahara
, Shingo Akiyoshi
, Yasushi Okazaki
, Isao Tanihata
, Yoshihide Hayashizaki^*

^*Corresponding author for this work

RIKEN
Japan Science and Technology Agency

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The construction of a genetic linkage map is the first, fundamental step to analyze the genetic properties of any organism. For this purpose, the restriction landmark genome scanning method (RLGS) can be used and has been shown to have high productivity in various genetic analyses. However, construction of a genetic linkage map by the RLGS method is laborious, because hundreds of spots must be scored, usually by visual observation. In order to reduce human involvement in the data processing, we developed an image analysis software, RAT (RLGS Analysis Tool). We evaluated its accuracy and feasibility by comparing the parental distribution patterns of RLGS spots obtained by RAT and by human observation, using Syrian hamster strain backcross progeny. We then used RAT to construct a genetic linkage map of the recombinant inbred strain SMXA. We were able to obtain 121 progenitor strain- specific spots that were assigned to a specific chromosome.

Original language	English
Pages (from-to)	611-616
Number of pages	6
Journal	Mammalian Genome
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/s003359901055
State	Published - 1999
Externally published	Yes

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title = "Application of the RLGS image analysis tool (RAT) to the construction of a genetic linkage map of recombinant inbred strain SMXA",

abstract = "The construction of a genetic linkage map is the first, fundamental step to analyze the genetic properties of any organism. For this purpose, the restriction landmark genome scanning method (RLGS) can be used and has been shown to have high productivity in various genetic analyses. However, construction of a genetic linkage map by the RLGS method is laborious, because hundreds of spots must be scored, usually by visual observation. In order to reduce human involvement in the data processing, we developed an image analysis software, RAT (RLGS Analysis Tool). We evaluated its accuracy and feasibility by comparing the parental distribution patterns of RLGS spots obtained by RAT and by human observation, using Syrian hamster strain backcross progeny. We then used RAT to construct a genetic linkage map of the recombinant inbred strain SMXA. We were able to obtain 121 progenitor strain- specific spots that were assigned to a specific chromosome.",

author = "Yuichi Sugahara and Shingo Akiyoshi and Yasushi Okazaki and Isao Tanihata and Yoshihide Hayashizaki",

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T1 - Application of the RLGS image analysis tool (RAT) to the construction of a genetic linkage map of recombinant inbred strain SMXA

AU - Sugahara, Yuichi

AU - Akiyoshi, Shingo

AU - Okazaki, Yasushi

AU - Tanihata, Isao

AU - Hayashizaki, Yoshihide

PY - 1999

Y1 - 1999

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AB - The construction of a genetic linkage map is the first, fundamental step to analyze the genetic properties of any organism. For this purpose, the restriction landmark genome scanning method (RLGS) can be used and has been shown to have high productivity in various genetic analyses. However, construction of a genetic linkage map by the RLGS method is laborious, because hundreds of spots must be scored, usually by visual observation. In order to reduce human involvement in the data processing, we developed an image analysis software, RAT (RLGS Analysis Tool). We evaluated its accuracy and feasibility by comparing the parental distribution patterns of RLGS spots obtained by RAT and by human observation, using Syrian hamster strain backcross progeny. We then used RAT to construct a genetic linkage map of the recombinant inbred strain SMXA. We were able to obtain 121 progenitor strain- specific spots that were assigned to a specific chromosome.

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Application of the RLGS image analysis tool (RAT) to the construction of a genetic linkage map of recombinant inbred strain SMXA

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