A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay

Ming Liang Zhang; Chang Qing Yi; Xia Fan; Kui Qing Peng; Ning Bew Wong; Meng Su Yang; Rui Qin Zhang; Shuit Tong Lee

doi:10.1063/1.2833695

A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay

Ming Liang Zhang
, Chang Qing Yi
, Xia Fan
, Kui Qing Peng
, Ning Bew Wong^*
, Meng Su Yang
, Rui Qin Zhang
, Shuit Tong Lee

^*Corresponding author for this work

City University of Hong Kong

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Large-scale uniform silicon nanowires (SiNWs) array was fabricated by chemical etching on n-Si (111) wafer. Silver nanoparticles (AgNPs) were loaded on their surfaces. The AgNPs on SiNWs (AgNPs@SiNWs) array exhibit strong surface-enhanced Raman effect. On the substrate surfaces, characteristic Raman signals are generated with trace amount of mouse immunoglobulin G (mIgG), goat-anti-mouse immunoglobulin G (gamIgG), and immune complexes formed from 4 ng each of mIgG and gamIgG. The shifted positions and changed intensities in Raman bands indicate the occurrence of immunoreactions. This AgNPs@SiNWs array is a unique substrate for surface-enhanced Raman spectroscopy to show the immune reagents and immunoreactions at higher sensitivity.

Original language	English
Article number	043116
Journal	Applied Physics Letters
Volume	92
Issue number	4
DOIs	https://doi.org/10.1063/1.2833695
State	Published - 2008
Externally published	Yes

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title = "A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay",

abstract = "Large-scale uniform silicon nanowires (SiNWs) array was fabricated by chemical etching on n-Si (111) wafer. Silver nanoparticles (AgNPs) were loaded on their surfaces. The AgNPs on SiNWs (AgNPs@SiNWs) array exhibit strong surface-enhanced Raman effect. On the substrate surfaces, characteristic Raman signals are generated with trace amount of mouse immunoglobulin G (mIgG), goat-anti-mouse immunoglobulin G (gamIgG), and immune complexes formed from 4 ng each of mIgG and gamIgG. The shifted positions and changed intensities in Raman bands indicate the occurrence of immunoreactions. This AgNPs@SiNWs array is a unique substrate for surface-enhanced Raman spectroscopy to show the immune reagents and immunoreactions at higher sensitivity.",

author = "Zhang, \{Ming Liang\} and Yi, \{Chang Qing\} and Xia Fan and Peng, \{Kui Qing\} and Wong, \{Ning Bew\} and Yang, \{Meng Su\} and Zhang, \{Rui Qin\} and Lee, \{Shuit Tong\}",

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doi = "10.1063/1.2833695",

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volume = "92",

journal = "Applied Physics Letters",

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TY - JOUR

T1 - A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay

AU - Zhang, Ming Liang

AU - Yi, Chang Qing

AU - Fan, Xia

AU - Peng, Kui Qing

AU - Wong, Ning Bew

AU - Yang, Meng Su

AU - Zhang, Rui Qin

AU - Lee, Shuit Tong

PY - 2008

Y1 - 2008

N2 - Large-scale uniform silicon nanowires (SiNWs) array was fabricated by chemical etching on n-Si (111) wafer. Silver nanoparticles (AgNPs) were loaded on their surfaces. The AgNPs on SiNWs (AgNPs@SiNWs) array exhibit strong surface-enhanced Raman effect. On the substrate surfaces, characteristic Raman signals are generated with trace amount of mouse immunoglobulin G (mIgG), goat-anti-mouse immunoglobulin G (gamIgG), and immune complexes formed from 4 ng each of mIgG and gamIgG. The shifted positions and changed intensities in Raman bands indicate the occurrence of immunoreactions. This AgNPs@SiNWs array is a unique substrate for surface-enhanced Raman spectroscopy to show the immune reagents and immunoreactions at higher sensitivity.

AB - Large-scale uniform silicon nanowires (SiNWs) array was fabricated by chemical etching on n-Si (111) wafer. Silver nanoparticles (AgNPs) were loaded on their surfaces. The AgNPs on SiNWs (AgNPs@SiNWs) array exhibit strong surface-enhanced Raman effect. On the substrate surfaces, characteristic Raman signals are generated with trace amount of mouse immunoglobulin G (mIgG), goat-anti-mouse immunoglobulin G (gamIgG), and immune complexes formed from 4 ng each of mIgG and gamIgG. The shifted positions and changed intensities in Raman bands indicate the occurrence of immunoreactions. This AgNPs@SiNWs array is a unique substrate for surface-enhanced Raman spectroscopy to show the immune reagents and immunoreactions at higher sensitivity.

UR - https://www.scopus.com/pages/publications/38849153720

U2 - 10.1063/1.2833695

DO - 10.1063/1.2833695

M3 - 文章

AN - SCOPUS:38849153720

SN - 0003-6951

VL - 92

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 4

M1 - 043116

ER -

A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay

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